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OxyELISA™ Oxidized Protein Quantitation Kit

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货号：S7250-1

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
Oxidized Protein M, H, R, Po, Eq, Rb, Gp, Ht, NhP, Ca ELISA Colorimetric
Description
Catalogue Number S7250
Trade Name
  • OxyELISA
Description OxyELISA™ Oxidized Protein Quantitation Kit
Overview The OxyELISA™ Oxidized Protein Quantitation Kit provides a sensitive methodology for detection and quantitation of proteins modified by oxygen free radicals and other reactive species. Oxygen-derived free radicals have been implicated in numerous physiological functions such as apoptosis, cancer, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases (for reviews, see ref. 1-4).

Oxygen free radicals are generated by environmental factors such as ionizing radiation and chemical substances. They are also produced during normal cellular metabolism by mitochondrial electron transport, the cellular redox system, and by immune responses (1, 5-7). Although cells have developed various antioxidant defenses to protect against free radicals (7-9), free radicals that escape the defenses attack and modify subcellular components – nucleic acids, lipids and proteins (1, 10-12). In some cases, cells respond to the oxidative stimuli and allow the organism to adapt to the oxidative stress (13-15).

Proteins are one of the major targets of oxygen free radicals and other reactive species. Oxidation of proteins modifies the side chains of methionine, histidine, and tyrosine and forms cysteine disulfide bonds (16-19). Metal catalyzed oxidation of proteins introduces carbonyl groups (aldehydes and ketones) at lysine, arginine, proline or threonine residues in a site-specific manner (1, 16, 20-22).

The oxidative modification of proteins can modulate biochemical characteristics of proteins such as enzymatic activity (21-23), DNA binding activities of transcription factors (24-26), and the susceptibility to proteolytic degradation (12, 25-28). While a relationship between protein oxidation and aging has been suggested (29-31), little is known about the importance of oxidative modification of individual proteins in the pathophysiology of free radical mediated processes.

In conclusion, the OxyELISA Oxidized Protein Quantitation Kit provides chemical and immunological reagents necessary to perform quantitative detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.

For Research Use Only; Not for use in diagnostic procedures
References
Product Information
Components
  • 2,4-Dinitrophenylhydrazine (DNPH) Solution
  • 1.1mL
    Oxidized Protein Standard (Lyophilized)
  • 1 vial
    Reduced Protein Standard (Lyophilized)
  • 1 vial
    5x Lysis Buffer/Protein Diluent
  • 20mL
    10x Blocking Buffer
  • 6mL
    10x Wash Buffer
  • 130mL
    Anti-DNP Mouse Monoclonal HRP Conjugated Antibody
  • 22μL
    3,3’,5,5’-Tetramethylbenzidine (TMB) Solution
  • 45mL
    Stop Solution
  • 12mL
    MILLEX® GP Syringe Driven Filter Unit (0.22μm)(Catalog No. SLGP033RS)
  • 3 Filters
    Adhesive Plate Sealing Films
  • 6 Films
    High Protein Binding Assay Plate
  • 2 Plates (96-well)
Detection method Colorimetric
Applications
Application The OxyELISA Oxidized Protein Quantitation Kit provides a sensitive methodology for detection & quantitation of proteins modified by oxygen free radicals & other reactive species.
Key Applications
  • ELISA
Biological Information
Species Reactivity
  • Mouse
  • Human
  • Rat
  • Pig
  • Horse
  • Rabbit
  • Guinea Pig
  • Hamster
  • Non-human Primate
  • Canine
Analytes Available
  • Oxidized Protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C for up to 1 year after date of receipt.
Packaging Information
Material Size 192 tests
Transport Information
Supplemental Information
Specifications

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