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Purified Human Apolipoprotein A-II

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货号：ALP20

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number ALP20
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Apolipoprotein A-II, human
Overview High Density Lipoprotein (HDL) isolated by sequential isopycnic ultracentrifugation are delipidated. Apo A-II is purified by column chromatography in 6M urea (Schonfeld et al. 1977). Purity is determined by quantitative densitometry of SDS polyacrylamide gels (Laemlli 1970). Protein is determined by the Pierce BCA method (which was found to be comparable to the Lowry method) with 1% BSA as the standard. Apo A-II, which is a dimer composed of identical subunits, has a Mr of 17 kD and a pI of 4.9. Apo-II activates hepatic lipase and inhibits LCAT. Apolipoproteins are soluble in aqueous solutions. However, due to its amphipathic structure Apo-II self-associates into oligomers at high protein concentrations. Apo A-II exists as dimers in buffers with 5M guanidine-HCL or 6M urea.

MOLECULAR WEIGHT:

17,400
Alternate Names
  • ApoAII
References
Product Information
Presentation Liquid in 10 mM NH4HCO3, pH 7.4. Non-sterile, no preservatives.
Applications
Key Applications
  • Positive Control
Biological Information
Concentration 4.89 mg/mL
Purity > 98%
Source Normal human plasma, fresh, non-frozen. Tested negative for HIV-1, HIV-2, HCV and HBc antibodies and HBsAg.
Entrez Gene Number
Entrez Gene Summary This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia.
Gene Symbol
  • APOA2
  • ApoA-II
  • Apo-AII
UniProt Number
UniProt Summary FUNCTION: SwissProt: P02652 # May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
SIZE: 100 amino acids; 11175 Da
SUBUNIT: Homodimer; disulfide-linked. Also forms a disulfide- linked heterodimer with APOD. Interacts with HCV core protein. Interacts with APOA1BP.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Plasma; synthesized in the liver and intestine.
SIMILARITY: SwissProt: P02652 ## Belongs to the apolipoprotein A2 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at < -20°C for up to six months. Maintain reconstituted Apo A-II at +4°C for up to 4 weeks. Freezing solutions of apolipoproteins in the absence of denaturants may favor increased self-association or aggregation.
Packaging Information
Material Size 500 µg
Transport Information
Supplemental Information
Specifications

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