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Qiagen 凯杰 Biosharp Omega

首页细胞生物学检测Millipore 密理博>

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Ready-to-Assay™ TP Prostanoid Receptor Frozen Cells.

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货号：HTS081RTA

品牌：Millipore 密理博

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Replacement Information

特卖

This product requires a User Agreement (Label License). Please see product datasheet for further information

重要规格表

Species Host Cells Protein Target
Human Chem-1 TP / TXA2 / PGH2
Description
Catalogue Number HTS081RTA
Trade Name
  • Ready-to-Assay
Description Ready-to-Assay™ TP Prostanoid Receptor Frozen Cells
Background Information Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

TP receptors (Thromboxane A2 receptors) are widely distributed among different organ systems and have been localized on both cell membranes and intracellular structures. TP receptors belong to G protein-coupled receptor family (Hirata et al., 1991). Activation of TP receptors induces platelet aggregation, vascular and respiratory smooth muscle constriction, and enhances mitogenic responses of vascular smooth muscle cells that are stimulated by growth factors (Ali et al., 1993; Hanasaki et al., 1990). The human TP receptor is encoded by a single gene that is alternatively spliced at the carboxyl terminus, resulting in two isoforms, TPα (343 residues) and TPα (407 residues). Both isoforms couple to Gq pathway, but couple oppositely to adenylate cyclase. Millipore’s cloned human TP-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant TP expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at TP.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection method Fluorescent
Applications
Application Human TP / TXA2 / PGH2 GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.
Key Applications
  • Calcium Flux Assays
  • cAMP Assays
Biological Information
Entrez Gene Number
GPCR Class A
Host Cells Chem-1
Protein Target TP / TXA2 / PGH2
Target Sub-Family Prostanoid
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 1 kit
Transport Information
Supplemental Information
Specifications

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