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Qiagen 凯杰 Biosharp Omega

首页细胞生物学检测Millipore 密理博>

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Ready-to-Assay™ EP3 Prostanoid Receptor Frozen Cells.

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货号：HTS092RTA

品牌：Millipore 密理博

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This product requires a User Agreement (Label License). Please see product datasheet for further information

重要规格表

Species Host Cells Protein Target
Human Chem-1 EP3
Description
Catalogue Number HTS092RTA
Trade Name
  • Ready-to-Assay
Description Ready-to-Assay™ EP3 Prostanoid Receptor Frozen Cells
Background Information Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Prostanoids are a series of arachidonic acid metabolites produced by the action of cyclooxygenase and subsequently by isomerases and synthases. Cells rapidly secrete prostanoids after synthesis, whereupon the prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostaglandin PGE2 causes pain, vasodilatation, immunosuppression of T cells, bone resorption and promotion of carcinogenesis. Four related GPCRs, EP1, EP2, EP3 and EP4, each bind to PGE2, but the different G protein coupling status of each receptor leads to distinct biological effects. Further diversity is generated by alternative splicing; the human gene for EP3 generates 9 alternatively spliced mRNAs encoding 8 isoforms of EP3 (Kotani et al., 1997). These isoforms of EP3 vary in sequence at their C-termini, and differ in their ability to couple to Gs, Gq or Gi (Kotani et al., 1995). EP3 is required for fever induced by pyrogens, a response long attributed to prostaglandins by the antipyretic action of aspirin and other COX inhibitors (Ushikubi et al., 1998). In animal models of allergy, PGE2-mediated activation of EP3 inhibits inflammation to counteract the allergy-promoting activity of PGE2 (Kunikata et al., 2005). Millipore’s cloned human EP3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant EP3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between EP3 and its ligands.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Applications
Application Human EP3 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Ligand Binding Assays
Biological Information
GPCR Class A
Host Cells Chem-1
Protein Target EP3
Target Sub-Family Prostanoid
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Place cells in liquid nitrogen immediately upon receipt.
Packaging Information
Material Size 1 kit
Transport Information
Supplemental Information
Specifications

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