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Qiagen 凯杰 Biosharp Omega

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384-Well PI3 Kinase Homogeneous Cell-Based Assay Kit

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货号：17-494-2

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Analytes Available Detection Methods
KA PI 3-Kinase HTRF, TR-Fret
Description
Catalogue Number 17-494
Description PIP3 Quantification TR-FRET Assay
Overview PIP3 Quantification TR-FRET Assay
References
Product Information
Components
  • Black 384-well plate
  • Detection Buffer
  • DM B
  • DM A
  • Stop B
  • PIP3 Standard
  • Lipid Recovery Buffer
  • 1M DTT
Detection method HTRF TR-Fret
Applications
Application The PI3 Kinase Homogenous Cell-Based Assay Kit TR-FRET provides an easy to use method for assaying PIP3 levels by detecting the displacement of biotin-PIP3 from an energy transfer complex.
Key Applications
  • Kinase Assay
Application Notes This assay requires lipid extraction from cells. A protocol is included with this assay.
Biological Information
Analytes Available
  • PI 3-Kinase
Entrez Gene Number
Entrez Gene Summary Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene Symbol
  • p110-alpha
  • MGC142161
  • PI3K
  • MGC142163
  • PIK3CA
UniProt Number
UniProt Summary FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 1 plate
Material Package Kit capacity: 384 wells
Transport Information
Supplemental Information
Specifications

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