A-SETA/CIN85/RUK/SH3KBPI

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IP, WB, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

IP, WB, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	05-733
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-SETA/CIN85/Ruk/SH3KBPI Antibody, clone 84

Description

Catalogue Number

05-733

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-SETA/CIN85/Ruk/SH3KBPI Antibody, clone 84

Product Information
Format	Purified
Presentation	Prtotein G purified antibody in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4

Product Information

Format

Purified

Presentation

Prtotein G purified antibody in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4

Applications
Application	Detect SETA/CIN85/Ruk/SH3KBPI using this Anti-SETA/CIN85/Ruk/SH3KBPI Antibody, clone 84 validated for use in IP, WB & IC.
Key Applications	Immunoprecipitation Western Blotting Immunocytochemistry

Applications

Application

Detect SETA/CIN85/Ruk/SH3KBPI using this Anti-SETA/CIN85/Ruk/SH3KBPI Antibody, clone 84 validated for use in IP, WB & IC.

Key Applications

Immunoprecipitation
Western Blotting
Immunocytochemistry

Biological Information
Immunogen	6His and HA tagged fusion protein corresponding to full length rat SETA
Clone	clone 84
Host	Mouse
Specificity	SETA
Isotype	IgG
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_031892
Entrez Gene Summary	CBL (MIM 165360) constitutively interacts with SH3 domain-containing proteins and, upon tyrosine phosphorylation, with SH2 domain-containing proteins. The SH3KBP1 gene encodes an 85-kD CBL-interacting protein that enhances tumor necrosis factor (MIM 191160)-mediated apoptotic cell death (Narita et al., 2001 [PubMed 11474197]).[supplied by OMIM]
Gene Symbol	SH3KBP1 CD2BP3 OTTHUMP00000061480 HSB-1 GIG10 CIN85 MIG18
Purification Method	Protein G Chromatography
UniProt Number	Q96B97
UniProt Summary	FUNCTION: SwissProt: Q96B97 # Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. SIZE: 665 amino acids; 73126 Da SUBUNIT: Can self-associate and form homotetramers. Interacts with CD2, F-actin capping protein, PIK3R3, GRB2, EGFR, MET, BLNK, MAP3K4, PDCD6IP, SPRY2, ARHGAP17, MAGI2, CRK, BCAR1, SOS1, DDEF1, CENTD3, HIP1R, SYNJ2, INPP5D and STAP1. Interacts with CBL and CBLB, but does not interact with CBLC. Two molecules of SH3KBP1 seem to bind through their respective SH3 1 domain to one molecule of CBLB. The interaction with CBL or CBLB and EGFR is increased upon EGF stimulation. The interaction with CBL is attenuated by PDCD6IP. Interacts through its proline-rich region with the SH3 domain of endophilins SH3GL1, SH3GL2 and SH3GL3. The SH3KBP1- endophilin complex seems to associate with a complex containing the phosphorylated receptor (EGFR or MET) and phosphorylated CBL. Probably associates with DDEF1 and phosphorylated EGFR. Probably part of a complex consisting of at least SH3KBP1, DDEF1 and CENTD3. Interacts with focal adhesion kinases PTK2 AND PTK2B, probably as a dimer. Interacts with DAB2 and probably associates with chathrin through its interaction with DAB2. Part of a complex consisting of SH3KBP1, DAB2, and clathrin heavy chain. DAB2 and clathrin dissociate from SH3KBP1 following growth factor treatment, enabling interaction with CBL. Interacts with DDN and probably associates with MAGI2 through its interaction with DDN. Interacts with the SH3 domains of SRC tyrosine-protein kinases SRC, LCK, LYN, FGR, FYN and HCK. Interacts with TRADD, BIRC2, TRAF1, TRAF2 and TNFR1, and the association with a TNFR1- associated complex upon stimulation with TNF-alpha seems to be mediated by SRC. Probably interacts with SH3KBP1. SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome; Peripheral membrane protein. Note=Localized in endocytic vesicles containing clustered receptors. Colocalizes with DDEF1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes (By similarity).TISSUE SPECIFICITY: Ubiquitously expressed. Also expressed in some cancer cell lines.DOMAIN:SwissProt: Q96B97 The SH3 domains mediate interaction with SHKBP1 (By similarity). PTM: Monoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus. SIMILARITY: Contains 3 SH3 domains.
Molecular Weight	85kDa

Biological Information

Immunogen

6His and HA tagged fusion protein corresponding to full length rat SETA

Clone

clone 84

Host

Mouse

Specificity

SETA

Isotype

IgG

Species Reactivity

Human
Mouse
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_031892

Entrez Gene Summary

CBL (MIM 165360) constitutively interacts with SH3 domain-containing proteins and, upon tyrosine phosphorylation, with SH2 domain-containing proteins. The SH3KBP1 gene encodes an 85-kD CBL-interacting protein that enhances tumor necrosis factor (MIM 191160)-mediated apoptotic cell death (Narita et al., 2001 [PubMed 11474197]).[supplied by OMIM]

Gene Symbol

SH3KBP1
CD2BP3
OTTHUMP00000061480
HSB-1
GIG10
CIN85
MIG18

Purification Method

Protein G Chromatography

UniProt Number

Q96B97

UniProt Summary

FUNCTION: SwissProt: Q96B97 # Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis.
SIZE: 665 amino acids; 73126 Da
SUBUNIT: Can self-associate and form homotetramers. Interacts with CD2, F-actin capping protein, PIK3R3, GRB2, EGFR, MET, BLNK, MAP3K4, PDCD6IP, SPRY2, ARHGAP17, MAGI2, CRK, BCAR1, SOS1, DDEF1, CENTD3, HIP1R, SYNJ2, INPP5D and STAP1. Interacts with CBL and CBLB, but does not interact with CBLC. Two molecules of SH3KBP1 seem to bind through their respective SH3 1 domain to one molecule of CBLB. The interaction with CBL or CBLB and EGFR is increased upon EGF stimulation. The interaction with CBL is attenuated by PDCD6IP. Interacts through its proline-rich region with the SH3 domain of endophilins SH3GL1, SH3GL2 and SH3GL3. The SH3KBP1- endophilin complex seems to associate with a complex containing the phosphorylated receptor (EGFR or MET) and phosphorylated CBL. Probably associates with DDEF1 and phosphorylated EGFR. Probably part of a complex consisting of at least SH3KBP1, DDEF1 and CENTD3. Interacts with focal adhesion kinases PTK2 AND PTK2B, probably as a dimer. Interacts with DAB2 and probably associates with chathrin through its interaction with DAB2. Part of a complex consisting of SH3KBP1, DAB2, and clathrin heavy chain. DAB2 and clathrin dissociate from SH3KBP1 following growth factor treatment, enabling interaction with CBL. Interacts with DDN and probably associates with MAGI2 through its interaction with DDN. Interacts with the SH3 domains of SRC tyrosine-protein kinases SRC, LCK, LYN, FGR, FYN and HCK. Interacts with TRADD, BIRC2, TRAF1, TRAF2 and TNFR1, and the association with a TNFR1- associated complex upon stimulation with TNF-alpha seems to be mediated by SRC. Probably interacts with SH3KBP1.
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome; Peripheral membrane protein. Note=Localized in endocytic vesicles containing clustered receptors. Colocalizes with DDEF1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes (By similarity).TISSUE SPECIFICITY: Ubiquitously expressed. Also expressed in some cancer cell lines.DOMAIN:SwissProt: Q96B97 The SH3 domains mediate interaction with SHKBP1 (By similarity).
PTM: Monoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus.
SIMILARITY: Contains 3 SH3 domains.

Molecular Weight

85kDa

Product Usage Statements
Quality Assurance	routinely evaluated by immunoblot on RIPA lysates from NG108-15 cells
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

routinely evaluated by immunoblot on RIPA lysates from NG108-15 cells

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

A-SETA/CIN85/RUK/SH3KBPI

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