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GP X Mu Opioid Receptor

市场价：
0.00
促销价：
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货号：AB5509

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R, Pm ICC, IHC Gp Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5509
Replaces AB1774
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Opioid Receptor µ Antibody
Alternate Names
  • MOR-1
References
Product Information
Format Affinity Purified
Presentation PBS containing 50% glycerol; Concentration 1.0 mg/ml
Applications
Application Anti-Opioid Receptor μ Antibody-AB5509 is an antibody against Opioid Receptor for use in IC, IH.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
Application Notes Immunohistochemistry: 1:50-500 See Protocol.

Immunocytochemistry: 1:50-500 See Protocol.

Optimal working dilutions must be determined by the end user.

IHC Protocol: Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5509. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

ICC Protocol: Mu opioid receptor transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum free media. Media was removed and cells were gently washed 3 times with serum free media. Following fixation, cells were processed for indirect immunofluorescence as described above.
Biological Information
Immunogen Synthetic peptide (Catalog number AG375) from the C-terminus of rat mu Opioid receptor corresponding to residues 384-398 of the rat mu opioid receptor.
Host Guinea Pig
Specificity Mu Opioid receptor.
Species Reactivity
  • Mouse
  • Rat
  • Primate
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • OPRM1
  • MOR-1
  • MOR1
  • OPRM
  • KIAA0403
UniProt Number
UniProt Summary FUNCTION: SwissProt: P35372 # Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.
SIZE: 400 amino acids; 44779 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P35372 ## Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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