APO-BRDU-50T

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Qiagen 凯杰 Biosharp Omega

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货号：APT115

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

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周一至周五：9:00-18:00

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0771-3293894

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重要规格表

Key Applications	Detection Methods
FC	Fluorescent

Description
Catalogue Number	APT115
Brand Family	Chemicon®
Trade Name	Apo-BrdU Chemicon
Description	Apo-BrdU™ Detection Kit
Overview	The CHEMICON APO-BRDU™ Kit is a two color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains the instructions and reagents required for measuring apoptosis in cells including; positive and negative control cells for assessing reagent performance; washing, reaction, and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and Propidium Iodide/RNase A solution for counter staining the total DNA.
Materials Required but Not Delivered	1. Flow Cytometer 2. Distilled Water 3. 1% (w/v) paraformaldehyde (methanol free) in PBS 4. 70% (v/v) ethanol 5. 37°C Water Bath 6. Ice Bucket 7. 12 x 75 mm flow cytometry test tubes 8. Micro-pipettor

References

Product Information
Components	The APO-BRDU™ Kit is shipped in one container and consists of two packages. One package is shipped at ambient temperature and should be stored at 2-8°C upon arrival. The other package is styrofoam containing frozen ice packs and the reagent contents should be stored at -20°C upon arrival. Note: Upon arrival store the reagents at the appropriate temperatures. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 60 cell suspensions including 5 mL positive and 5 mL negative control cell suspensions of approximately 1 x 106 cells per mL in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described on page 6. Positive Control Cells, Brown cap Negative Control Cells, White cap Wash Buffer, Blue cap Reaction Buffer, Green cap TdT Enzyme, Yellow cap Br-dUTP, Violet cap Rinsing Buffer, Red cap Fluorescein~PRB-1 mAb, Orange cap PI/RNase Staining Buffer, Amber bottle
Detection method	Fluorescent

Product Information

Components

The APO-BRDU™ Kit is shipped in one container and consists of two packages. One package is shipped at ambient temperature and should be stored at 2-8°C upon arrival. The other package is styrofoam containing frozen ice packs and the reagent contents should be stored at -20°C upon arrival. Note: Upon arrival store the reagents at the appropriate temperatures.
Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 60 cell suspensions including 5 mL positive and 5 mL negative control cell suspensions of approximately 1 x 106 cells per mL in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described on page 6.
Positive Control Cells, Brown cap
Negative Control Cells, White cap
Wash Buffer, Blue cap
Reaction Buffer, Green cap
TdT Enzyme, Yellow cap
Br-dUTP, Violet cap
Rinsing Buffer, Red cap
Fluorescein~PRB-1 mAb, Orange cap
PI/RNase Staining Buffer, Amber bottle

Detection method

Fluorescent

Applications
Application	The kit measures apoptosis in cells.
Key Applications	Flow Cytometry

Biological Information
Species Reactivity	All

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Precautions and Warnings 1. The components of this kit are for Research Use only and are not intended for diagnostic procedures. 2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid skin contact, wash immediately with water. See Material Safety Data Sheets. 3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.

Storage and Shipping Information

Storage Conditions

Precautions and Warnings

1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.

2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid skin contact, wash immediately with water. See Material Safety Data Sheets.

3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.