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| Replacement Information |
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| Species Reactivity | Detection Methods |
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| A Broad Range Of Species | Fluorometric |
| Description | |
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| Overview | Suitable for the in vitro detection of caspase-3 activity in cell lysates. The assay is simple, rapid, and includes all controls. Suitable for use in frozen samples. Assay is validated against the Nucleosome ELISA Kit (Cat. No. QIA25) and the Cell Death Detection NMP ELISA Kit (Cat. No. QIA20). A high-throughput drug-screening version is also available (Cat. No. HTS02). |
| Catalogue Number | QIA70 |
| Brand Family | Calbiochem® |
| Materials Required but Not Delivered | • 1 M dithiothreitol (DTT, Cleland regent) solution (1.54 g to 10 ml H2O) made fresh (can be stored at -20°C for one month). • 2-20 µl, 20-200 µl and 200-1000 µl precision pipettes with disposable tips. • Fluorescent plate reader capable of measuring excitation ~400 nm, and emission at ~505 nm. • A centrifuge, for spinning tubes or 96-well plate and to pulse-spin the Recombinant Caspase-3 vials. • Microcentrifuge tubes. • Protease inhibitor cocktail set III (Cat. No. 539134) Note: It is recommended to add protease inhibitor cocktail at a 1:500 dilution to Sample Buffer and Assay Buffer just before use. These protease inhibitors have been shown to exhibit no effect on recombinant caspase-3 activity. |
| References | |
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| References | X. Sun, et al. 1999. J. Biol. Chem. 274, 5053. N.A. Thornberry and Y. Lazebnik, 1998. Science 281, 1312. S. J. Martin and D. 1995 R. Green, Cell 82, 349. |
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| Biological Information | |
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| Assay time | 2.5-3 h |
| Sample Type | Cell lysates |
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