VEGF165, Human, Recombinant

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VEGF165, Human, Recombinant

市场价：: 0.00

促销价：: ¥0.00

货号：PF074-10UG

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

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工作时间

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0771-3293894

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Replacement Information

重要规格表

Purity
≥95% by SDS-PAGE

Description
Overview	Recombinant, human VEGF₁₆₅ (a 165 amino acid splice varian of VEGF) expressed under serum-free conditions in Spodoptera frugiperda cells and purified via a proprietary sequential chromatrography methods. VEGF₁₆₅ binds to the connective tissue growth factor (CTGF) and the resulting complex has no angiogenic activity. Degradation of CTGF by matrix metalloproteinases results in VEGF-induced angiogenesis during embryonic development and pathological disease states. The recombinant disulfide-linked homodimeric human VEGF is glycosylated and, thus, migrates as a ~42 kDa protein in SDS-PAGE under non-reducing conditions.
Catalogue Number	PF074
Brand Family	Calbiochem®
Synonyms	Vascular Permeability Factor

References
References	Hashimoto, G., et al. 2002. J. Biol. Chem. In Press. Soker, S., et al. 1998. Cell 92, 735. Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521. Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699. Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303. Brown, L., et al. 1992. Kidney International 42, 1457. Dvorak, H., et al. 1991. J. Exp. Med. 174, 1275. Conn, G., et al. 1990. PNAS 87, 1323. Leung, D.W., et al. 1989. Science 246, 1306.

References

Hashimoto, G., et al. 2002. J. Biol. Chem. In Press.
Soker, S., et al. 1998. Cell 92, 735.
Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
Brown, L., et al. 1992. Kidney International 42, 1457.
Dvorak, H., et al. 1991. J. Exp. Med. 174, 1275.
Conn, G., et al. 1990. PNAS 87, 1323.
Leung, D.W., et al. 1989. Science 246, 1306.

Product Information
Form	Lyophilized
Formulation	Carrier free.
Preservative	None

Applications
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Application Notes	Proliferation Studies

Biological Information
Biological activity	EC₅₀ of 1-6 ng/ml as determined by the dose dependent proliferation of human ubilical vein epithelial cells (HUVEC)
Purity	≥95% by SDS-PAGE

Physicochemical Information
Contaminants	Endotoxins: ≤100 pg/µg VEGF

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.