MMP-3, Proenzyme, Human, Recombinant

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

Qiagen 凯杰 Biosharp Omega

客服热线 0771-3293894

首页重组蛋白Millipore 密理博>

图片仅供参考，请以实物为准

MMP-3, Proenzyme, Human, Recombinant

市场价：: 0.00

促销价：: ¥0.00

货号：PF063-10UG

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

3720.00

Plasmid Mini Kit I(200) 200...

576.00

10ul吸头，透明，袋装，可灭菌...

85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

Replacement Information

重要规格表

Description
Overview	Recombinant, human pro-MMP-3 purified from cell culture supernatant. May be used as a positive control or standard for zymographic analysis, or substrate assay. Requires activation for immunoblotting, prior to use. M.W. 57000/58000.
Catalogue Number	PF063
Brand Family	Calbiochem®

References
References	Sole, S., et al. 2004. J. Neuropathol. Exp. Neurol. 63, 338. Galazka, G., et al. 1996. Biochem. 35, 11221. Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861. Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434. Netzel-Arnett, S., et al. 1991. Anal. Biochem. 195, 86. Woessner, J.F. 1991. FASEB J. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99. Zymography References Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259. Kleiner, D.E. and Stetler-Stevenson W.G. 1994. Anal. Biochem. 218, 325. Heussen, C. and Dowdle, E.B. 1980. Anal. Biochem. 102, 196. Substrate Cleavage Assay References Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259. Netzel-Arnett, S., et al. 1991. Anal. Biochem. 195, 86.

References

Sole, S., et al. 2004. J. Neuropathol. Exp. Neurol. 63, 338.
Galazka, G., et al. 1996. Biochem. 35, 11221.
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Netzel-Arnett, S., et al. 1991. Anal. Biochem. 195, 86.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.

Zymography References
Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259.
Kleiner, D.E. and Stetler-Stevenson W.G. 1994. Anal. Biochem. 218, 325.
Heussen, C. and Dowdle, E.B. 1980. Anal. Biochem. 102, 196.

Substrate Cleavage Assay References
Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259.
Netzel-Arnett, S., et al. 1991. Anal. Biochem. 195, 86.

Product Information
Activity	The activity of proenzyme MMP 3 was measured by substrate cleavage assay using 0.5 mM thiopeptiolide (Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-Oet) as a substrate. The activity was also assessed by degradation of a peptide substrate (DNP-PYAYWMR) using activated MMP-3 as measured by HPLC.
EC number	3.4.24.17
Form	Lyophilized
Formulation	Lyophilized from 100 mM NaCl, 50 mM HEPES, pH 7.3.

Applications
Application References	ZYMOGRAPHY REFERENCES Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259. Kleiner, D.E. and Stetler-Stevenson W.G. 1994. Anal. Biochem. 218, 325. Heussen, C. and Dowdle, E.B. 1980. Anal. Biochem. 102, 196. SUBSTRATE CLEAVAGE ASSAY REFERENCE Xia, T., et al. 1996. Biochim. Biophys. Acta. 1293, 259. Netzel-Arnett S, et al. 1991. Anal. Biochem. 195, 86.
Key Applications	Immunoblotting (Western Blotting) Substrate Cleavage Assay Zymography
Application Notes	Immunoblotting (see comments) Substrate Cleavage Assay (see comments) Zymography (see comments)
Application Comments	Proenzyme MMP-3 may be used as a positive control or standard for immunoblotting, zymographic analysis, or substrate cleavage assays. 0.5 μg/lane was used for SDS-PAGE and immunoblotting. For zymography with casein 1μg/lane of Proenzyme MMM-3 or activated MMP-3 was used. Proenzyme MMP-3 can be activated in vitro by incubation in 50 mM Tris, pH 7.5, containing 0.05% Triton-X-100, 5 mM CaCl₂ and 1 mM 4 aminophenyl mercuric acetate (APMA) for 2-4 hours at 37°C. To dissolve APMA, make a 10 mM stock solution in 0.05 M NaOH. Approximately 90% of proenzyme MMP-3 is activated with a 4 hour incubation at 37°C using 1 mM APMA.

Biological Information
Purity	≥95% by SDS-PAGE

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot into siliconized vials and freeze (-70°C).