Recombinant, human heregulin expressed in E. coli. Heregulins have been shown to be mitogenic for Schwann cells and various epithelial cells. It is recommended that the protein be titrated for optimal results in individual systems.
Catalogue Number
PF048
Brand Family
Calbiochem®
Synonyms
NDF, Neu Differentiation Marker
References
References
Riese, D.J., et al. 1996. J. Biol. Chem.271, 20047. Holmes, W.E., et al. 1992. Science256, 1205. Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Product Information
Form
Lyophilized
Formulation
Lyophilized from PBS, 50 µg BSA/µg cytokine.
Applications
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Application Notes
Proliferation Studies
Application Comments
It is recommended that the protein be titrated for optimal results in individual systems.
Biological Information
Biological activity
EC₅₀ ~20-40 ng/ml as determined by a cell proliferation assay using breast carcinoma cell line SK-BR-3 or in a serum-free cell proliferation assay using the human cell line MCF7.
Purity
≥97% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Store lyophilized protein dessicated at -20°C. Lyophilized product should be reconstituted in sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 50 µg/ml. Following initial thaw, aliquot and freeze (-20°C).