Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent.
Supplied with a directional insert. Note: 1 KU = 1000 units; 1 MU = 1,000,000 units.
Bioassay: 350-450 units/mg Hygromycin B. 106 units is equivalent to ~900 mg.
Catalogue Number
400051
Brand Family
Calbiochem®
References
References
Dean, N. 1995. Proc. Natl. Acad. Sci. USA92, 1287. Hamada, W., et al. 1994. Curr. Genetics 26, 251. Hubbard, S. C., et al. 1994. J. Biol. Chem.269, 3717. Rikkerink, E. H., et al. 1994. Current Genetics25, 202. Sugimoto, K., et al. 1994. Plant J.5, 863. Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol.66, 2731. Gaken, J., et al. 1992. Biotechniques13, 32. Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932. Ma, H., et al. 1992. Gene117, 161. MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol.276, 67. Norman, J.A., et al. 1992. Mol. Pharmacol.41, 53. Bilang, R., et al. 1991. Gene100, 247. Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA88, 10558. Lee, M.G-S., and Van der Ploeg, L. 1991. Gene105, 255. Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol.57, 1423. MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother.35, 2125. Zhou, J., et al. 1991. Gene107, 307. Bulte, L., and Bennoun, P. 1990. Current Genetics18, 155. Giordano, T.J., and McAllister, W.T. 1990. Gene88, 285. Salauze, D., et al. 1990. Antimicrob. Agents Chemother.24, 1915. Carrasco, L., et al. 1989. Pharmacol. Ther.9, 311. Crespi, C.L., et al. 1989. Carcinogenesis10, 295. Damm, B., et al. 1989. Mol. Gen. Genetics217, 6. Egelhoff, T.T., et al. 1989. Mol. Cell. Biol.9, 1965. Kronstad, J.W., et al. 1989. Gene79, 97. Perlin, D.S., et al. 1988. J. Biol. Chem.263, 118. Cullen, D., et al. 1987. Gene57, 21. Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459. Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun.117, 6. Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.
Product Information
CAS number
31282-04-9
Form
Amber to brown liquid
Hill Formula
C₂₀H₃₇N₃O₁₃
Chemical formula
C₂₀H₃₇N₃O₁₃
Structure formula Image
Applications
Biological Information
Biological activity
The activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
Primary Target
growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
Secondary target
protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA
Purity
>85% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
WK2130000
Safety Information
R Phrase
R: 26/27/28-37/38-41
Very toxic by inhalation, in contact with skin and if swallowed. Irritating to respiratory system and skin. Risk of serious damage to eyes.
S Phrase
S: 23-26-36/37/39-45
Do not breathe fumes. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Highly Toxic & Carcinogenic / Teratogenic
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Aqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.
Note: This product is highly toxic. Read enclosed MSDS before handling.
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