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| Replacement Information |
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| Empirical Formula | CAS # |
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| C₃₅H₂₈N₄O₅ | 154589-96-5 |
| Description | |
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| Overview | A cell-permeable ESCs (Embryonic Stem Cells) EMT (Epithelial-Mesenchymal Transition) inducer that is reported to prime/sensitize human and murine ESC cultures for much more enhanced/efficient differentiation into progenitor cells of multiple lineages, including Activin A- (Cat. No. 114700) induced definitive endoderm differentiation (60% differentiation from murine R1 ESCs with 5 ng/ml Activin A and 200 nM Spd), ATRA- (Cat. No. 554720) induced neuronal progenitor differentiation, and BMP-4- (Cat. No. 203642) induced mesoderm differentiation, under suboptimal lineage specifying stimuli concentrations and often serum-free conditions, while Spd sensitization alone does not induce any ESCs differentiation. Spd is shown to interact with NME2/DNPK B/PUF and prevent NME2 nuclear localization and c-myc transcription in murine R1 ESC cultures (46% and 85% drop in c-myc mRNA in 24 h, respectively, with 200 nM and 500 nM Spd), which accounts for, at least in part, its EMT induction activity. Although a structural analog of the broad-spectrum kinase inhibitors Staurosporine (Cat. Nos. 569396 and 569397) and UCN-01 (Cat. No. 539644), Spd exhibits significant kinase inhibitory activities only at elevated concentrations, but not at concentrations ≤500 nM typically used in EMT induction. |
| Catalogue Number | 569393 |
| Brand Family | Calbiochem® |
| Synonyms | ESC EMT Inducer, Spd |
| References | |
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| References | Zhu, S., et al. 2009. Cell Stem Cell 4, 416. |
| Product Information | |
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| CAS number | 154589-96-5 |
| Form | Yellow solid |
| Hill Formula | C₃₅H₂₈N₄O₅ |
| Chemical formula | C₃₅H₂₈N₄O₅ |
| Structure formula Image | |
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| Biological Information | |
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| Purity | ≥95% by HPLC |
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| S Phrase | S: 22-24/25 Do not breathe dust. Avoid contact with skin and eyes. |
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| Packaged under inert gas | Packaged under inert gas |
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