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sFRP-3, His•Tag®, Mouse, Recombinant

市场价：: 0.00

促销价：: ¥0.00

货号：565834-10UG

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Purity
≥95% by SDS-PAGE

Description
Overview	Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag^® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
Catalogue Number	565834
Brand Family	Calbiochem®

References
References	Hoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.

Product Information
Form	Lyophilized
Formulation	Lyophilized from 0.2 µm filtered PBS, BSA.

Applications

Biological Information
Biological activity	Proliferation Assay: ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells. Functional ELISA: 50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Purity	≥95% by SDS-PAGE

Physicochemical Information
Contaminants	Endotxin: ≤1 EU/µg

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze
Special Instructions	Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

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