Topoisomerase I, His•Tag®, Human, Wild Type, Recombinant, S. frugiperda - 炼石商城

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Topoisomerase I, His•Tag®, Human, Wild Type, Recombinant, S. frugiperda

市场价：: 0.00

促销价：: ¥0.00

货号：614850-1SET

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Description
Overview	Recombinant, human topoisomerase I fused to a His•Tag^® sequence and expressed in S. frugiperda insect cells. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. Since Topo I can cause large amounts of single-strand DNA breaks it is an important target for antitumor agents. One unit= 1 ng protein.
Catalogue Number	614850
Brand Family	Calbiochem®
Synonyms	Topo I, Wild Type

References
References	Pommier, Y., et al. 1998. Biochim. Biophys. Acta 1400, 83. Pourquier, P., et al. 1997. J. Biol. Chem. 272, 26441. Rossi, F., et al. 1996. Nature 381, 80. Sekiguchi, J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 785. Giovanella, B.C., et al. 1989. Science 246, 1046. Wang, J,C. 1985. Annu. Rev. Biochem. 54, 665.

Product Information
Form	Liquid
Formulation	5000 U enzyme in 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9 and 1 vial dilution buffer (100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0).

Applications

Biological Information
Biological activity	0.1-1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 µl reaction.
Purity	≥95% by SDS-PAGE
Specific Activity	1 unit/ng protein
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw aliquot and freeze (-70°C). For storage of diluted protein, dilute in storage buffer with 500µg/ml molecular biology grade BSA, aliquot, and freeze (-70°C).

Packaging Information

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Supplemental Information

Specifications

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