Enzymatic component of the holotoxin. Has both NAD-glycohydrolase and ADP-ribosyltransferase activities. Inhibits neutrophil-mediated inflammation in vivo via its ADP-ribosylation activity. Unable to penetrate cells in the absence of the B oligomer.
Catalogue Number
516854
Brand Family
Calbiochem®
References
References
Brito, G.A., et al. 1997. Infect. Immun. 65, 1114. Gill, D.M., and Coburn, J., 1988. Biochim. Biophys. Acta954, 65. Kaslow, H.R., and Lesikar, D.D., 1987. Biochemistry26, 4397. Burns, D.L., and Manclark, C.R., 1986. J. Biol. Chem.261, 4324. Moss, J., et al. 1983. J. Biol. Chem.258, 11879. Tamura, M., et al. 1982. Biochemistry21, 5516.
Product Information
ATP Competitive
N
Form
Lyophilized
Formulation
Lyophilized from 10 mM Tris, 100 µM EDTA, 0.04% CHAPS, pH 8.0.
Reversible
N
Applications
Biological Information
Primary Target
Has both NAD-glycohydrolase and ADP-ribosyltransferase activities
Purity
Single major band by SDS-PAGE
Physicochemical Information
Cell permeable
N
Contaminants
≤0.1% holotoxin by CHO cell assay
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 23/24/25
Toxic by inhalation, in contact with skin and if swallowed.
S Phrase
S: 36/37/39-45
Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Toxic
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C). Stock solutions, prepared from high ionic strength buffers, are stable for up to 3 months at 4°C.
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
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