A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1, S-1 (A protomer) is responsible for the enzymatic activity of the toxin, and S-2, S-3, S-4, and S-5 form the B oligomer which is responsible for the toxins binding to the cell surface. NOTE: This product is not activated, hence for use in cell-free systems, activation is required.
Catalogue Number
516562
Brand Family
Calbiochem®
Synonyms
Islet-activating Protein
References
References
Fryer, M.W. 1992. Neurosci. Lett.146, 84. Gierschik, P. 1992. Curr. Topics Microbiol. Immunol.175, 69. Harada, J., et al. 1992. Eur. J. Pharmacol.227, 301. Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem.267, 13735. Kaslow, H.R., and Burns, D.L. 1992. FASEB J.6, 2684. Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap.263, 1479. Kaslow, H.R., et al. 1987. Biochemistry26, 123. Hewlett, E.L., et al. 1983. Infect. Immun.40, 1198. Moss, J., et al. 1983. J. Biol. Chem.258, 11879.
Product Information
CAS number
70323-44-3
ATP Competitive
N
Form
Salt-free lyophilized solid
Reversible
N
Applications
Biological Information
Biological activity
≤0.01 ng/ml. The activity is defined as the lowest concentration that promotes a clustering pattern in the CHO cell assay
Primary Target
ADP-ribosylation of guanine nucleotide-binding regulatory proteins
Purity
Five distinct bands by SDS-PAGE
Physicochemical Information
Cell permeable
N
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
XW5883750
Safety Information
R Phrase
R: 23/24/25
Toxic by inhalation, in contact with skin and if swallowed.
S Phrase
S: 36/37/39-45
Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Toxic
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+2°C to +8°C
Do not freeze
Yes
Special Instructions
Following reconstitution, store in the refrigerator (4°C). For long term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for up to 6 months for use in CHO cell assays and for up to 2 months for use in non-cellular systems.
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.