A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1, S-1 (A protomer) is responsible for the enzymatic activity of the toxin, and S-2, S-3, S-4, and S-5 form the B oligomer which is responsible for the toxins binding to the cell surface. NOTE: This product is not activated, hence for use in cell-free systems, activation is required.
Catalogue Number
516561
Brand Family
Calbiochem®
Synonyms
Islet-activating Protein
References
References
Fryer, M.W. 1992. Neurosci. Lett.146, 84. Gierschik, P. 1992. Curr. Topics Microbiol. Immunol.175, 69. Harada, J., et al. 1992. Eur. J. Pharmacol.227, 301. Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem.267, 13735. Kaslow, H.R., and Burns, D.L. 1992. FASEB J.6, 2684. Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap.263, 1479 Kaslow, H.R., et al. 1987. Biochemistry26, 123. Hewlett, E.L., et al. 1983. Infect. Immun.40, 1198. Moss, J., et al. 1983. J. Biol. Chem.258, 11879.
Product Information
CAS number
70323-44-3
Activity
≤35 pmol/min/µg in the presence of 1 µM calmodulin
ATP Competitive
N
Form
Liquid
Formulation
In 500 mM NaCl, 50 mM Tris, 10 mM glycine, 50% glycerol, pH 7.5.
Reversible
N
Applications
Biological Information
Biological activity
≤1 ng/ml as determined in a clustering assay using CHO cells line
Primary Target
Guanine nucleotide-binding regulatory proteins
Primary Target IC<sub>50</sub>
≤1 ng/ml as determined in a clustering assay using CHO cells line
Purity
Five distinct bands by SDS-PAGE
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Cell permeable
N
Contaminants
Adenylate cyclase.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
XW5883750
Safety Information
R Phrase
R: 23/24/25
Toxic by inhalation, in contact with skin and if swallowed.
S Phrase
S: 36/37/39-45
Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Toxic
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
A uniform suspension may be achieved by gentle mixing prior to use.
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
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