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Protein Phosphatase, Lambda, Recombinant, E. coli

市场价：: 0.00

促销价：: ¥0.00

货号：539514-20KU

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

Description
Overview	Recombinant λ PPase expressed in E. coli. A 221-amino acid, Mn²⁺-dependent, protein phosphatase. Hydrolyzes phosphate groups on serine, threonine, tyrosine, or histidine residues. Inhibited by orthovanadate. Note: 1KU = 1,000 units.
Overview	Note: 1 KU = 1000 units.
Catalogue Number	539514
Brand Family	Calbiochem®
Synonyms	Protein Phosphatase, Lambda

References
References	Zhuo, S., et al. 1993. J. Biol. Chem. 268, 17754. Gordon, J.A. 1991. Methods Enzymol. 201, 477. Cohen, P.T.W. and Cohen, P. 1989. Biochem. J. 260, 931.

Product Information
Activity	≥400,000 units/ml.
Unit of Definition	One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of <i>p</i>NPP per min at 30°C, pH 7.8. 20-200 Units will typically remove >95% of phosphates from serine/threonine residues in 0.1 nmol of protein in 30 min. 100-1000 Units will typically remove >95% of phosphates from tyrosine in 0.1 nmol of protein in 30 min.
Form	Liquid
Formulation	In 100 mM NaCl, 50 mM HEPES, 2 mM DTT, 0.1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.01% BRIJ^® 35 Detergent, pH 7.5.

Applications

Biological Information
Purity	≥95% by SDS-PAGE

Physicochemical Information
Contaminants	Protease activity: none detected

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).

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