PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

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PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

市场价：: 0.00

促销价：: ¥0.00

货号：539481-100KU

品牌：Millipore 密理博

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北京朝阳

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数量：

EA (预计3-5工作日到货)

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Replacement Information

Description
Overview	Recombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca²⁺ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
Catalogue Number	539481
Brand Family	Calbiochem®
Synonyms	cAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit

References
References	Grant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022. Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553. Adams, J.A., et al. 1995. Biochemistry 34, 2447. Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242. Brindle, P., et al. 1993. Nature 364, 821. Hajnoczky, G., et al. 1993. Biochem. J. 293, 413. Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135. Wang, L-Y., et al. 1993. Science 259, 1173. Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940. Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300. Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.

References

Grant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
Adams, J.A., et al. 1995. Biochemistry 34, 2447.
Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
Brindle, P., et al. 1993. Nature 364, 821.
Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
Wang, L-Y., et al. 1993. Science 259, 1173.
Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.

Product Information
Activity	≥2.5 x 10⁶ U/ml
Unit of Definition	One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; <a href ="/Products/ProductDisplay.asp→catNO=05-23-4900">Cat. No. 05-23-4900</a>) per min at 30°C, pH 7.5.
EC number	2.7.1.37
Form	Liquid
Formulation	In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.

Applications

Biological Information

Physicochemical Information
Contaminants	Phosphatases, proteases: none detected

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).