Recombinant, human tumor necrosis factor-α expressed in E. coli. A cytokine produced primarily by activated macrophages that activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein kinase (SAPK).
Catalogue Number
654205
Brand Family
Calbiochem®
Synonyms
rhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
References
References
Merck Index 13, 9882. Tsuchida, H., et al. 1995. J. Immunol. 154, 2403. Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689. Polunovsky, V.A., et al. 1994. Exp. Cell Res.214, 584. Koide, M., et al. 1993. FEBS Lett.318, 213. Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem.266, 7313.
Product Information
CAS number
94948-59-1
Form
Lyophilized
Formulation
Carrier-free
Applications
Biological Information
Biological activity
ED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤0.1 ng/µg TNF-α
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
MT6435000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
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