A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin and 1 ml of resuspension buffer.
Catalogue Number
650277
Brand Family
Calbiochem®
References
References
Henzel, W.J., et al. 1993. PNAS90, 5011. Rice, R.H., et al. 1977. Biochim Biophys Acta492, 316.
Product Information
Unit of Definition
One BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
Form
Lyophilized
Applications
Biological Information
Specific Activity
≥7500 BAEE U/mg
Physicochemical Information
Contaminants
Chymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 36/37/38-42
Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation.
S Phrase
S: 22-24-26-36/37-45
Do not breathe dust. Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
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