Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units.
Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units.
Catalogue Number
6502
Brand Family
Calbiochem®
References
References
Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17. Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem.239, 1799. Hummel, B.C.W. 1955. Can. J. Biochem. Physiol.37, 1393.
Product Information
CAS number
9002-07-7
Activity
≥2,500 USP units/mg dry weight
Unit of Definition
One USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
EC number
3.4.21.4
Form
Solid
Formulation
Salt-free
Hygroscopic
Hygroscopic
Applications
Biological Information
Physicochemical Information
Contaminants
Chymotrypsin: ≤50 NF units/mg dry weight
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
YN5075000
Safety Information
R Phrase
R: 36/37/38-42
Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation.
S Phrase
S: 22-24-26-36/37
Do not breathe dust. Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
-20°C
Hygroscopic
Hygroscopic
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.
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