Recombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
Catalogue Number
375230
Brand Family
Calbiochem®
Synonyms
Hepatopoietin A
References
References
Corso, S., et al. 2005. Trends Mol. Med.11, 284. Karihaloo, A., et al. 2005. Nephron Exp. Nephrol.100, e40. Urbanek, K., et al. 2005. Circ. Res.97, 663. Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol.286, 21. Rosario, M. and W. Birchmeier. 2004. Dev. Cell7, 3. Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci.100, 12039. Chiu, S.J., et al. 2002. J. Biomed. Sci.9, 261. Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun.299, 472. Maeshima, A., et al. 2000. Kid. Int.58, 1511. Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol.258, 501. Saelman, E.U.M., et al. 1995. J. Cell Sci.108, 3531. Mineo, R., et al. 1994. Endocrinology145, 4355. Mizuno, K., et al. 1994. J. Biol. Chem.269, 1131. Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun.199, 772. Montesano, R., et al. 1991. Cell67, 901.
Product Information
Form
Solid
Formulation
Lyophilized from 0.2 µm sterile-filtered PBS, BSA.
Applications
Application
HGF, Mouse, Recombinant Recombinant, consists of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
Biological Information
Biological activity
Cell Proliferation: ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.
Functional ELISA KD <0.2 nM as measured by binding to C-Met (HGF Receptor).
Purity
≥95% by SDS-PAGE as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions.
Physicochemical Information
Contaminants
Endotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
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