Recombinant, mouse leptin espressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations in the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. Suppresses insulin secretion by inhibiting activities of Ca2+-dependent PKC isoforms. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis.
Catalogue Number
429705
Brand Family
Calbiochem®
Synonyms
βrmOB
References
References
Anderwald, C., et al. 2002. Mol. Endocrinol.16, 1612. Ookuma, M., et al. 1998. Diabetes 47, 219. Campfield, L.A., et al. 1995. Science269, 546. Halaas, J.L., et al. 1995. Science269, 543. Pelleymounter, M.A., et al. 1995. Science 269, 540. Zhang, Y., et al. 1994. Nature 372, 425.
Product Information
CAS number
181030-10-4
Form
Lyophilized
Formulation
Lyophilized from a sterile filtered PBS.
Applications
Biological Information
Biological activity
ED₅₀ = 0.2-1 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
Purity
≥97% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1.0 EU/µg leptin
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
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