Recombinant, human leptin expressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations of the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism, and body temperature. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis. Note: Following complete dissolution in 15 mM HCl, add 7.5 mM sterile NaOH and bring the pH to approximately 5.2.
Catalogue Number
429700
Brand Family
Calbiochem®
Synonyms
rhOB
References
References
Anderwald, C., et al. 2002. Mol. Endocrinol.16, 1612. Ookuma, M., et al. 1998. Diabetes 47, 219. Campfield, L.A., et al. 1995. Science 269, 546. Halaas, J.L., et al. 1995. Science 269, 543. Pelleymounter, M.A., et al. 1995. Science 269, 540. Zhang, Y., et al. 1994. Nature372, 425.
Product Information
CAS number
177404-21-6
Form
Lyophilized
Formulation
Lyophilized from a sterile filtered solution in PBS.
Applications
Biological Information
Biological activity
ED₅₀ = 0.4-2 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
Purity
≥97% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1.0 EU/µg leptin
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
To reconstitute lyophilized leptin, add 15 mM sterile HCl (0.5 ml/1 mg vial or 2.5 ml/5 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1mg vial or 1.5 ml/5 mg vial) to bring the pH to ~5.2. Lyophilized samples are stable for at least six months at -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 4°C for one month or at -70°C for three months without detectable loss of activity. Avoid repeated freeze/thaw cycles of reconstituted solutions.
新浪微博
腾讯微博
QQ空间
客服
