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PERK, GST-Fusion, Human, Recombinant, E. coli

市场价：: 0.00

促销价：: ¥0.00

货号：324881-5UG

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

Description
Overview	Recombinant, human PERK consisting of amino acids 563-1115 fused at the N-terminus to GST and expressed in E. coli. PERK phosphorylates the α-subunit of eukaryotic translation initiation factor 2 (EIF2α), which leads to its inactivation and reduces translation initiation. The active kinase is useful for the study of PERK regulation and for inhibitor screening.
Overview	Note: the calculated MW is 88,000, but the protein runs at ~115,000 on SDS-PAGE.
Catalogue Number	324881
Brand Family	Calbiochem®
Synonyms	EIF2AK3 PKR
Application Data	The activity of PERK was measured as outlined in the Recommended Reaction Conditions.

References
References	Baltzis, D., et al. 2007. J Biol Chem. 43, 31675. Koumenis, C., et al. 2002. Mol Cell Biol. 21, 7405.

Product Information
Unit of Definition	Kinase activity is measured as the molar amount of phosphate incorporated into SMAD3 at 30°C using a final concentration of 50 µM ATP.
Form	Liquid
Formulation	In 50 mM Tris-HCl, 50 mM NaCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.

Applications

Biological Information
Purity	≥90% by SDS-PAGE
Specific Activity	Please refer to the lot-specific Certificate of Analysis
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).

Packaging Information

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Supplemental Information

Specifications

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