Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Acts as a survival factor in preventing apoptosis.
Catalogue Number
324831
Brand Family
Calbiochem®
Synonyms
Epidermal Growth Factor, Human, Recombinant, E. coli
References
References
Merlo, G.R., et al. 1995. J. Cell Biol. 128, 1185. Yoshida, T., et al. 1993. Brain Res. Dev.76, 147. Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA88, 415. Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69. Downward, J., et al. 1984. Nature311, 483. Gray, A., et al. 1983. Nature303, 722. Scott, J., et al. 1983. Science221, 236. Cohen, S. 1960. Proc. Natl. Acad. Sci. USA46, 302.
Product Information
CAS number
62229-50-9
Form
Solid
Formulation
Lyophilized from sterile-filtered PBS.
Applications
Biological Information
Biological activity
ED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: <1.0 EU/μg EGF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
KB4518000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.
新浪微博
腾讯微博
QQ空间
客服
