Native, neutral endopeptidase from porcine kidney. A cell surface, zinc containing metallopeptidase that is abundant in the brush border membrane of the kidney proximal tubules. Also present in brain, endothelial cells, liver, and lung. Peptidase activity is specifically directed toward the cleavage on the amino side of hydrophobic residues. Contains an arginine in the active site that can interact with the C-terminal carboxylate of substrates.
Catalogue Number
324762
Brand Family
Calbiochem®
Synonyms
CD10, Common Acute Lymphocytic Antigen, Endopeptidase-24.11, Enkephalinase, Microvillus Proteinase, Neprilysin
References
References
Barnes, K., et al. 1995. J. Neurochem. 64, 1826. Gafford, J.T., et al. 1983. Biochemistry 22, 3265.
Product Information
Unit of Definition
One unit of enzyme will liberate 1.0 µmol <i>p</i>NA per min from Glutaryl-(Ala)₂-Phe-<i>p</i>NA at 25°C, pH 7.4.
EC number
3.4.24.11
Form
Lyophilized
Formulation
Lyophilized from 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, TRITON® X-100 Detergent, pH 7.4.
Applications
Biological Information
Purity
≥95% by HPLC
Specific Activity
≥850 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 days at 4°C.
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
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