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Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

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号:324717-200MIU

牌:Millipore 密理博

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      塑胶安瓿;塑胶针药瓶 200 miu
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      Description
      Overview Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
      Note: 1 mU = 1 milliunit.
      Catalogue Number 324717
      Brand Family Calbiochem®
      Synonyms Endo-β-N-acetylglucosaminidase H, Endo H
      References
      References Rao, V., et al. 1995. Structure 3, 449.
      Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
      Trumbly, R.J., et al. 1985. J. Biol. Chem. 260, 5683.
      Product Information
      CAS number 52769-51-4
      Activity ≥3 units/ml
      Unit of Definition One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.
      EC number 3.2.1.96
      Form Liquid
      Formulation In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
      Applications
      Biological Information
      Specific Activity ≥30 units/mg protein
      Physicochemical Information
      Contaminants N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

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