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Casein Kinase II, Human, Recombinant, E. coli

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号:218701-10KU

牌:Millipore 密理博

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EA (预计3-5工作日到货)

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Replacement Information
Description
Overview Recombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits (M.W. 44 kDa) and regulatory β-subunits (M.W. 26 kDa) in an α2β2-tetramer. This material corresponds to one of several forms of native CKII. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies.
Note: 1 KU = 1000 units.
Catalogue Number 218701
Brand Family Calbiochem®
Synonyms rhCKII
References
References Yamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.
Product Information
Activity ≥500,000 units/ml
Unit of Definition One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.
Form Liquid
Formulation In 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON® X-100 Detergent, pH 7.5.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special Instructions Following initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

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