Recombinant, human galectin-3 expressed in E. coli and subsequently cleaved with Clostridium histolyticum collagenase type VII to yield galectin-3C. The resulting galectin-3C is purified by affinity chromatography. Galectin-3C is a truncated form of galectin-3 that contains the carbohydrate binding domain but lacks the 107 amino acids at the N-terminus of galectin-3. It is suggested to play a key role in autoimmune diseases, allergic reactions, inflammation, tumor cell metastasis, atherosclerosis, and diabetes.
Catalogue Number
345832
Brand Family
Calbiochem®
References
References
Hoyer K.K., et al. 2004. Am. J. Pathol.164, 893. John C.M., et al. 2003. Clin. Cancer Res.9, 2374. Iurisci, I., et al. 2000. Clin Cancer Res6, 1389. Van Den Brule, F. A., et al. 2000. Int. J. Cancer89, 361. Inohara, H. 1999. Cancer85, 2475. Nakamura, M., et al. 1999. Int. J. Oncol.15, 143. Idikio, H., et al. 1998. Int. J. Oncol.12, 1287. Ochieng, J., et al. 1998. Biochim. Biophys. Acta.1379 97. Castronovo, V., et al. 1996. J. Pathol.179, 43. Liu, F.T., et al. 1996. Biochemistry35 6073. Schoeppner, H. L., et al. 1995. Cancer75, 2818. Massa, S.M., et al. 1993. Biochemistry32, 260. Oda, Y., et al. 1991. Gene99, 279.
Product Information
Form
Lyophilized
Formulation
Lyophilized from deionized water, 2µg lactose/µg lectin.
Applications
Biological Information
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤ 1 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions (in the presence of a molar excess of lactose) are stable for up to 4 months at -20°C. For use in carbohydrate binding assays the solution should be dialyzed to remove the lactose stabilizer.