ATG3 (Human Recombinant)

Description
Catalogue Number	03-217
Brand Family	Chemicon®
Trade Name	Chemicon
Description	ATG3 (Human) Recombinant Protein
Background Information	Autophagy works as a cellular housekeeper by eliminating defective proteins and organelles, the prevention of abnormal protein aggregate accumulation, and the removal of intracellular pathogens. Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3, which displays the same enzymatic characteristics in vitro as yeast Apg3, is thought to be an autophagy-related ubiquitin carrier protein (E2)-like enzyme that is the go between for the conjugation on ATG8 and phosphatidylethanolamine (PE).

Description

Catalogue Number

03-217

Brand Family

Chemicon®

Trade Name

Chemicon

Description

ATG3 (Human) Recombinant Protein

Background Information

Autophagy works as a cellular housekeeper by eliminating defective proteins and organelles, the prevention of abnormal protein aggregate accumulation, and the removal of intracellular pathogens. Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3, which displays the same enzymatic characteristics in vitro as yeast Apg3, is thought to be an autophagy-related ubiquitin carrier protein (E2)-like enzyme that is the go between for the conjugation on ATG8 and phosphatidylethanolamine (PE).

Product Information
Components	1 vial of 20 µg Human ATG3 recombinant protein in 20 mM Tris-HCl pH 8.0, containing 150 mM NaCl, protease inhibitor cocktail and 10% glycerol.

Product Information

Components

1 vial of 20 µg
Human ATG3 recombinant protein in 20 mM Tris-HCl pH 8.0, containing 150 mM NaCl, protease inhibitor cocktail and 10% glycerol.

Applications
Application Notes	Purified Human ATG3 protein gel 0.05 µg of Human ATG3 recombinant protein was loaded onto 10-20% Bis-Tris gel and stained with ponceau.

Applications

Application Notes

Purified Human ATG3 protein gel
0.05 µg of Human ATG3 recombinant protein was loaded onto 10-20% Bis-Tris gel and stained with ponceau.

Biological Information
Concentration	See datasheet for lot-specific concentration.
Purity	≥99% (SDS-PAGE)
Gene Symbol	ATG3 APG3 APG3L
UniProt Number	Q9NT62
UniProt Summary	Function: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Subunit structure: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Interacts with FNBP1L. Subcellular Location: Cytoplasm Tissue specificity: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. Post-translational modification: Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis. Sequence similarities: Belongs to the ATG3 family.
Molecular Weight	~40 kDa observed. Calculated molecular weight of 36 kDa.

Biological Information

Concentration

See datasheet for lot-specific concentration.

Purity

≥99% (SDS-PAGE)

Gene Symbol

ATG3
APG3
APG3L

UniProt Number

Q9NT62

UniProt Summary

Function: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Subunit structure: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Interacts with FNBP1L.
Subcellular Location: Cytoplasm
Tissue specificity: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Post-translational modification: Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis.
Sequence similarities: Belongs to the ATG3 family.

Molecular Weight

~40 kDa observed.
Calculated molecular weight of 36 kDa.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at -80ºC for up to 3 months after date of receipt. Avoid freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Store at -80ºC for up to 3 months after date of receipt.
Avoid freeze/thaw cycles.

Packaging Information
Material Size	20 µg

Packaging Information

Material Size

20 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

ATG3 (Human Recombinant)

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