Beclin 1 (Human Recombinant)

Description
Catalogue Number	03-231
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Beclin 1 (Human) Recombinant Protein
Background Information	The Beclin-1 (BECN1) gene encodes a coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.

Description

Catalogue Number

03-231

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Beclin 1 (Human) Recombinant Protein

Background Information

The Beclin-1 (BECN1) gene encodes a coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.

Product Information
Components	1 vial of 20 µg Human Beclin 1 recombinant protein in 20 mM Tris-HCl pH 8.0, containing 150 mM NaCl, protease inhibitor cocktail and 10% glycerol.

Product Information

Components

1 vial of 20 µg
Human Beclin 1 recombinant protein in 20 mM Tris-HCl pH 8.0, containing 150 mM NaCl, protease inhibitor cocktail and 10% glycerol.

Applications
Application Notes	Western Blot Analysis A 1:1000 dilution of Cat. No. MABC139, Anti-Beclin-1, clone 4E10.1 was used to detect 0.05 µg of Human Beclin-1 recombinant protein (Cat. No. 03-231). Proteins were visualized using a Goat Anti-Mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Purified Human Beclin 1 protein gel 0.05 µg of Human Beclin 1 recombinant protein was loaded onto 10-20% Bis-Tris gel and stained with ponceau.

Applications

Application Notes

Western Blot Analysis
A 1:1000 dilution of Cat. No. MABC139, Anti-Beclin-1, clone 4E10.1 was used to detect 0.05 µg of Human Beclin-1 recombinant protein (Cat. No. 03-231). Proteins were visualized using a Goat Anti-Mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Purified Human Beclin 1 protein gel
0.05 µg of Human Beclin 1 recombinant protein was loaded onto 10-20% Bis-Tris gel and stained with ponceau.

Biological Information
Concentration	See datasheet for lot-specific concentration.
Purity	≥99% (SDS-PAGE)
Gene Symbol	BECN1 GT197
UniProt Number	Q14457
UniProt Summary	Function:Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Subunit structure: Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy By similarity. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB By similarity. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1. Subcellular location: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells Tissue specificity: Ubiquitous. Post-translational modification: Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.Polyubiquitinated by NEDD4, both with 'Lys11'- and 'Lys63'-linkages. 'Lys'-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. Sequence similarities: Belongs to the beclin family.
Molecular Weight	~56 kDa observed. Uncharacterized bands may be observed. Calculated molecular weight of 52 kDa.

Biological Information

Concentration

See datasheet for lot-specific concentration.

Purity

≥99% (SDS-PAGE)

Gene Symbol

BECN1
GT197

UniProt Number

Q14457

UniProt Summary

Function:Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Subunit structure: Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy By similarity. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB By similarity. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1.
Subcellular location: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells
Tissue specificity: Ubiquitous.
Post-translational modification: Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.Polyubiquitinated by NEDD4, both with 'Lys11'- and 'Lys63'-linkages. 'Lys'-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.
Sequence similarities: Belongs to the beclin family.

Molecular Weight

~56 kDa observed.
Uncharacterized bands may be observed.
Calculated molecular weight of 52 kDa.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at -80ºC for up to 3 months after date of receipt. Avoid freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Store at -80ºC for up to 3 months after date of receipt.
Avoid freeze/thaw cycles.

Packaging Information
Material Size	20 µg

Packaging Information

Material Size

20 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Beclin 1 (Human Recombinant)

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