PluriSTEM® -XF Recombinant Vitronectin - 炼石商城

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PluriSTEM® -XF Recombinant Vitronectin

市场价：: 987.00

促销价：: ¥0.00

货号：CC130

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Key Applications
Cell Attachment, CULT, Cult

Description
Catalogue Number	CC130
Trade Name	PluriSTEM
Description	PluriSTEM-XF™ Recombinant Vitronectin
Alternate Names	S-protein Serum-spreading factor V75
Background Information	PluriSTEM™ Recombinant Vitronectin is an effective xeno-free alternative to matrigel™ and has been validated to successully culture human ES and iPS cells using PluriSTEM™ Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media. PluriSTEM™ Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

References

Product Information
Presentation	Product is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol.

Applications
Application	Human pluripotent stem cell culture.
Key Applications	Cell Attachment Cell Culture Stem Cell Culture
Application Notes	Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

Biological Information
Concentration	0.5 mg/mL
Host	E. coli
Purity	Product is ≥ 95% pure by SDS-PAGE.
Gene Symbol	VTN
UniProt Number	P04004
Stem Cell Type	Human Embryonic Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles.

Packaging Information
Material Size	500 µg

Transport Information

Supplemental Information

Specifications

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