Human Recombinant Hp1a (CBX5)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, Vrt	WB, IF	Rb	Culture Supernatant	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, Vrt

WB, IF

Culture Supernatant

Monoclonal Antibody

Description
Catalogue Number	05-1059
Description	Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal
Alternate Names	CSAID-binding protein Csaids binding protein Cytokine suppressive anti-inflammatory drug-binding protein MAP kinase MXI2 MAP kinase p38 alpha MAX-interacting protein 2 Mitogen-activated protein kinase p38 alpha cytokine suppressive anti-inflammatory drug binding protein mitogen-activated protein kinase 14 p38 MAP kinase p38 mitogen activated protein kinase p38alpha Exip stress-activated protein kinase 2A
Background Information	The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Description

Catalogue Number

05-1059

Description

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal

Alternate Names

CSAID-binding protein
Csaids binding protein
Cytokine suppressive anti-inflammatory drug-binding protein
MAP kinase MXI2
MAP kinase p38 alpha
MAX-interacting protein 2
Mitogen-activated protein kinase p38 alpha
cytokine suppressive anti-inflammatory drug binding protein
mitogen-activated protein kinase 14
p38 MAP kinase
p38 mitogen activated protein kinase
p38alpha Exip
stress-activated protein kinase 2A

Background Information

The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Product Information
Format	Culture Supernatant
Presentation	Rabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide.

Product Information

Format

Culture Supernatant

Presentation

Rabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide.

Applications
Application	Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.
Key Applications	Western Blotting Immunofluorescence

Applications

Application

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.

Key Applications

Western Blotting
Immunofluorescence

Biological Information
Immunogen	Synthetic peptide corresponding to amino acids surrounding and including phosphorylated Thr180/Tyr182 of human p38α.
Epitope	Phosphorylated Thr180/Tyr182
Clone	8.78.8
Host	Rabbit
Specificity	This antibody detects p38α when phosphorylated on Thr180/Tyr182.
Isotype	IgG
Species Reactivity	Human Mouse Vertebrates
Species Reactivity Note	Human and Mouse tested. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001306
Entrez Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
Gene Symbol	P38B P38BETA2 PRKM11 SAPK2 SAPK2B p38-2 p38Beta p38b
Modifications	Phosphorylation
UniProt Number	Q15759
UniProt Summary	FUNCTION: Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium By similarity. ENZYME REGULATION: Activated by phosphorylation on threonine and tyrosine by MKK6. Inhibited by pyridinyl-imidazole related compounds. TISSUE SPECIFICTY: Highest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas. DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme Probable. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Molecular Weight	38 kDa

Biological Information

Immunogen

Synthetic peptide corresponding to amino acids surrounding and including phosphorylated Thr180/Tyr182 of human p38α.

Epitope

Phosphorylated Thr180/Tyr182

Clone

8.78.8

Host

Rabbit

Specificity

This antibody detects p38α when phosphorylated on Thr180/Tyr182.

Isotype

IgG

Species Reactivity

Human
Mouse
Vertebrates

Species Reactivity Note

Human and Mouse tested. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001306

Entrez Gene Summary

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Gene Symbol

P38B
P38BETA2
PRKM11
SAPK2
SAPK2B
p38-2
p38Beta
p38b

Modifications

Phosphorylation

UniProt Number

Q15759

UniProt Summary

FUNCTION: Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium By similarity.
ENZYME REGULATION: Activated by phosphorylation on threonine and tyrosine by MKK6. Inhibited by pyridinyl-imidazole related compounds.
TISSUE SPECIFICTY: Highest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme Probable.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.

Molecular Weight

38 kDa

Product Usage Statements
Quality Assurance	Evaluated by western blot on anisomycin-treated HeLa cell lysate. Western Blot Analysis: 1:5,000-1:10,000 dilution of this antibody was used to detect p38 phosphorylated on Thr180/Tyr182 in anisomycin-treated HeLa and NIH/3T3 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by western blot on anisomycin-treated HeLa cell lysate.
Western Blot Analysis: 1:5,000-1:10,000 dilution of this antibody was used to detect p38 phosphorylated on Thr180/Tyr182 in anisomycin-treated HeLa and NIH/3T3 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Human Recombinant Hp1a (CBX5)

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