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Wnt-1, Human Recombinant

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货号：GF175

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Source Purity
Cult E. coli. Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue Number GF175
Description WNT-1, Human Recombinant
Alternate Names
  • WNT1
  • Proto-oncogene Wnt-1
  • Proto-oncogene Int-1 homolog
Background Information Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
References
Product Information
Presentation Product is filtered through a 0.2 micron filter before lyophilization. Product is presented in .
Applications
Key Applications
  • Stem Cell Culture
Biological Information
Purity Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Source E. coli.
Cross Reactivity Mouse
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Bioactivity assay: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Store at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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