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Sonic HedgeHog (Shh), Human Recombinant

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促销价：
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货号：GF174

品牌：Millipore 密理博

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Replacement Information

重要规格表

Source Purity
E. coli. Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue Number GF174
Description Sonic HedgeHog (Shh) Protein, Human Recombinant
Alternate Names
  • SHH
  • HHG-1
Background Information The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development. Shh is expressed in key embryonic tissues such as the Hensen’s node, the zone of polarizing activity in the posterior limb bud, the notochord, and the floor plate of the neural tube. Shh is involved in regulating the patterning of the developing central nervous system, somite, and limb. Shh plays an important role in the development of particular tissues such as whisker, hair, foregut, tooth and bone. Evidence also suggests that Shh is involved in regulating stem cell fates of neural and hematopoietic lineages, and that aberrant Shh signaling is implicated in basal cell carcinomas and other diseases. Shh genes are highly conserved and have been identified in a variety of species including human, mouse, frog, fish, and chicken. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
References
Product Information
Presentation Product is filtered through a 0.2 micron filter before lyophilization.
Applications
Application The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene.
Biological Information
Purity Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Source E. coli.
Cross Reactivity None
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Bioactivity assay: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Store at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size 25 µg
Transport Information
Supplemental Information
Specifications

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