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ANG-1, Human Recombinant

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货号：GF164

品牌：Millipore 密理博

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Replacement Information

重要规格表

Source Purity
Hela Cells Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue Number GF164
Description ANG-1 Protein, Human Recombinant
Alternate Names
  • ANG1
  • Angiopoietin1
  • Hepatic fibrinogen/angiopoietin-related protein
  • PPARG angiopoietin related protein
  • angiopoietin-like 4
  • angiopoietin-like 4 protein
  • angiopoietin-related protein 4
Background Information Angiopoietin1 (Ang1) and Angiopoietin2 (Ang2) are two closely related secreted ligands which bind with similar affinity to Tie2,
a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1/Tie-2 signaling appears to be regulated by Angiopoietin-2 (Ang-2), a natural antagonist for Tie-2 that exerts its effects through an internal autocrine loop mechanism. In addition to suppressing endothelial cell activation by inhibiting the expression of adhesion and inflammatory molecules, Ang-1 enhances endothelial cell survival and capillary morphogenesis, and lessens capillary permeability. As such, Ang-1 has a potential to become an effective therapeutic agent for treating various endothelium disorders, including several severe human pulmonary diseases. Recombinant human ANG-1, derived from HeLa cells, is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 – 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein.
References
Product Information
Presentation Product is filtered through a 0.2 micron filter before lyophilization.
Applications
Application The Ang-1 protein & Ang-2 protein are secreted ligands which bind with similar affinity to Tie2, a receptor tyrosine kinase with immunoglobulin & epidermal growth factor homology domains expressed on endothelial & early hematopoietic cells.
Biological Information
Purity Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses
Source Hela Cells
Cross Reactivity None
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Bioactivity assay: Determined by its ability to induce adhesion of human umbilical vein endothelial cells (HUVEC).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Store at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size 20 µg
Transport Information
Supplemental Information
Specifications

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