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PDGF-BB, Human Recombinant Animal Free

价:
0.00
价:
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号:GF310

牌:Millipore 密理博

账期 货到付款

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Source Purity
E. coli Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue Number GF310
Description PDGF-BB Protein, Human Recombinant Animal Free
Alternate Names
  • Platelet Derived Growth Factor-BB
  • Platelet Derived Growth Factor
  • PDGF-2
  • Platelet-derived growth factor B chain
  • Platelet-derived growth factor beta polypeptide
  • Proto-oncogene c-Sis
Background Information Platelet derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the αgranules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGFAA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
References
Product Information
Formulation
Presentation Product is filtered through a 0.2 micron filter before lyophilization.
Applications
Application The Platelet derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. Recombinant animal free human PDGF-BB is manufactured using all non-animal reagents.
Biological Information
Purity Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Source E. coli
Cross Reactivity Cow, Monkey, Mouse, Pig, Rat
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Store at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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