0
| Replacement Information |
|---|
| Key Applications |
|---|
| Track single cell and cell population migration (migration assay, cell culture) |
| Description | |
|---|---|
| Catalogue Number | MMA205 |
| Trade Name |
|
| Description | Millicell® µ-Migration Assay Kit |
| Overview | Cell migration occurs in a variety of biological processes including wound healing, embryonic development, and immune response. Because of the importance of cell migration in angiogenesis, oncology, neurology, and especially immunology, there is an increased interest in understanding the underlying biological mechanisms at a single cell level. Traditional multiwell (Boyden chamber) migration assays can have limitations, such as: (1) gradients are not linear, well-established, or controlled, (2) quantification of migratory cells is based on an endpoint measurement rather than a dynamic determination, and (3) imaging of the cells while they are migrating is currently not possible. The Millicell µ-Migration Assay Kit overcomes these limitations. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours. Designed for video microscopy assays, the µ-Migration Slide is made from a plastic with high optical qualities similar to those of glass. At specific time intervals, images of the observation area can be acquired, allowing real-time monitoring and quantitative measurements of cell migration. Benefits
How the Kit Works For detailed videos and instructions on the use of the µ-migration kit, please visit: Millicell® µ-Migration Assay Kit For instructions on how to access and use the free Image-J plug-in software to analyze the data and images collected using the µ-Migration Assay Kit, please visit: Millicell® µ-Migration Assay Kit. |
| Materials Required but Not Delivered | 1. Adherent cells 2. Inverted Microscope (phase contrast, fluorescence, etc.) 3. Heating stage 4. Time-lapse video equipment (CCD camera, video camera, acquisition software) 5. Motorized stage and autofocus (x,y,z) (optional) 6. Precision pipettes and pipette tips (10 µL and 200 µL) 7. Fisherbrand Micropipette 0.1-10 µL Tips 8. Accutase™ Cell Dissociation Solution (Catalog. No. SCR005) 9. Cell culture growth medium 10. Phosphate-Buffered Saline (1X PBS) (Catalog No. BSS-1005-B) 11. Sterile distilled water (Cat. No. TMS-006-C or TMS-006-B) 12. Low speed centrifuge 13. Sterile microcentrifuge tubes 14. 37ºC Incubator with 5% CO2 15. Scepter™ Handheld Automated Cell Counter (Cat No. PHCC20040 or PHCC20060) or Hemacytometer 16. Trypan Blue (optional) |
| References |
|---|
| Biological Information | |
|---|---|
| Species Reactivity |
|
| Physicochemical Information |
|---|
| Dimensions |
|---|
| Materials Information |
|---|
| Toxicological Information |
|---|
| Safety Information according to GHS |
|---|
| Safety Information |
|---|
| Packaging Information | |
|---|---|
| Material Size | 1 Kit |
| Transport Information |
|---|
| Supplemental Information |
|---|
| Specifications |
|---|
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！
服务热线
0771-3293894
新浪微博
腾讯微博
QQ空间
客服
