Millicell® µ-Angiogenesis Activation Assay

Key Applications	Detection Methods
ACT, CULT	Fluorescent

Key Applications

Detection Methods

ACT, CULT

Fluorescent

Description
Catalogue Number	MMA130
Trade Name	Millicell
Description	Millicell® µ-Angiogenesis Activation Assay Kit
Overview	Overview Studying how compounds affect angiogenesis, either to promote or inhibit new capillary tube formation can lead to therapies affecting wound healing, tissue regeneration, cardiovascular disease, stroke, tumor progression, and more. The Millicell μ-Angiogenesis activation and inhibition kits provide a powerful, quantitative platform for real-time monitoring of changes in tubule formation with unprecedented optical resolution. Benefits Continuous, live monitoring of cells as tubules form Even, flat slide surface prevents any liquid meniscus from forming, eliminating out-of-focus areas Full well visualization at low magnification Optimized assay conditions in a miniaturized format Compatible with multi-channel pipettes and fixation reagents Less reagents and cells needed, reducing costs and waste by up to 80% All-in-one slide used to grow, assay, and stain cells Studying how compounds affect angiogenesis, either to promote or inhibit new capillary tube formation, can lead to therapies affecting wound healing, tissue regeneration, cardiovascular disease, stroke, tumor progression, and more. The Millicell® μ-Angiogenesis activation and inhibition assay kits provide a powerful, quantitative platform for real-time monitoring of changes in tubule formation with unprecedented optical resolution. Applications Angiogenesis Assays For more information on Cell Culture & Systems visit: www.emdmillipore.com/cellculture
Background Information	Angiogenesis, the formation of new blood vessels from a pre-existing vascular network, occurs normally in development and is critical for a majority of the vessel formation that occurs during embryogenesis, tissue generation, and wound healing. However, abnormal blood vessel growth, either excessive or insufficient, can be the underlying cause of many deadly and debilitating diseases including cancer, cardiovascular disease, stroke, and diabetic and age-related blindness. Identification of specific compounds that promote or inhibit angiogenesis may provide promising treatments for these diseases. Tubular formation is a multi-step process involving cell adhesion, migration, differentiation, and growth. Millipore’s Millicell® µ-Angiogenesis Inhibition Assay provides an efficient system for the rapid and accurate identification of factors that inhibit tube formation of endothelial cells. The µ-Angiogenesis slide, with enhanced optical capabilities in a microscale format, allows for real time and continuous monitoring of tube formation in a simplifed and reproducible manner.
Materials Required but Not Delivered	1. Inverted Light Microscope 2. Fluorescent microscope (if Calcein AM is used) 3. Precision pipettes 4. Accutase™ Cell Dissociation Solution (Catalog. No. SCR005) 5. Human umbilical vein endothelial cells (HUVEC) (Catalog No. SCCE001) or any other experimental cell lines capable of tube formation. 6. EndoGRO™ LS Complete Medium (Cat. No. SCME001) or other endothelial cell basal medium. 7. Phosphate-Buffered Saline (1X PBS) (Catalog No. BSS-1005-B) 8. Sterile distilled water (Cat. No. TMS-006-C or TMS-006-B) 9. Low speed centrifuge 10. Sterile eppendorf tubes 11. Precision pipette tips. We recommend 0.1–10 mL TipOne filter tips (USA Scientific Catalog No. 1121-3810) or Rainin’s 20 µL Precision Pipette Tips (Rainin Catalog No. RT-L10S) 12. 37ºC Incubator with 5% CO2 13. Hemacytometer 14. Trypan Blue 15. DMSO

Description

Catalogue Number

MMA130

Trade Name

Millicell

Description

Millicell® µ-Angiogenesis Activation Assay Kit

Overview

Overview

Studying how compounds affect angiogenesis, either to promote or inhibit new capillary tube formation can lead to therapies affecting wound healing, tissue regeneration, cardiovascular disease, stroke, tumor progression, and more. The Millicell μ-Angiogenesis activation and inhibition kits provide a powerful, quantitative platform for real-time monitoring of changes in tubule formation with unprecedented optical resolution.
Benefits

Continuous, live monitoring of cells as tubules form
Even, flat slide surface prevents any liquid meniscus from forming, eliminating out-of-focus areas
Full well visualization at low magnification
Optimized assay conditions in a miniaturized format
Compatible with multi-channel pipettes and fixation reagents
Less reagents and cells needed, reducing costs and waste by up to 80%
All-in-one slide used to grow, assay, and stain cells

Studying how compounds affect angiogenesis, either to promote or inhibit new capillary tube formation, can lead to therapies affecting wound healing, tissue regeneration, cardiovascular disease, stroke, tumor progression, and more. The Millicell® μ-Angiogenesis activation and inhibition assay kits provide a powerful, quantitative platform for real-time monitoring of changes in tubule formation with unprecedented optical resolution.

Applications

Angiogenesis Assays

For more information on Cell Culture & Systems visit: www.emdmillipore.com/cellculture

Background Information

Angiogenesis, the formation of new blood vessels from a pre-existing vascular network, occurs normally in development and is critical for a majority of the vessel formation that occurs during embryogenesis, tissue generation, and wound healing. However, abnormal blood vessel growth, either excessive or insufficient, can be the underlying cause of many deadly and debilitating diseases including cancer, cardiovascular disease, stroke, and diabetic and age-related blindness. Identification of specific compounds that promote or inhibit angiogenesis may provide promising treatments for these diseases.

Tubular formation is a multi-step process involving cell adhesion, migration, differentiation, and growth. Millipore’s Millicell® µ-Angiogenesis Inhibition Assay provides an efficient system for the rapid and accurate identification of factors that inhibit tube formation of endothelial cells. The µ-Angiogenesis slide, with enhanced optical capabilities in a microscale format, allows for real time and continuous monitoring of tube formation in a simplifed and reproducible manner.

Materials Required but Not Delivered

1. Inverted Light Microscope
2. Fluorescent microscope (if Calcein AM is used)
3. Precision pipettes
4. Accutase™ Cell Dissociation Solution (Catalog. No. SCR005)
5. Human umbilical vein endothelial cells (HUVEC) (Catalog No. SCCE001) or any other experimental cell lines capable of tube formation.
6. EndoGRO™ LS Complete Medium (Cat. No. SCME001) or other endothelial cell basal medium.
7. Phosphate-Buffered Saline (1X PBS) (Catalog No. BSS-1005-B)
8. Sterile distilled water (Cat. No. TMS-006-C or TMS-006-B)
9. Low speed centrifuge
10. Sterile eppendorf tubes
11. Precision pipette tips. We recommend 0.1–10 mL TipOne filter tips (USA Scientific Catalog No. 1121-3810) or Rainin’s 20 µL Precision Pipette Tips (Rainin Catalog No. RT-L10S)
12. 37ºC Incubator with 5% CO2
13. Hemacytometer
14. Trypan Blue
15. DMSO

Product Information
Components	1. Millicell® µ-Angiogenesis Slides: (Part No. CS203030) Five tissue culture treated slides. Each slide contains 15 wells. 2. Fibrinogen Solution: (Part No. CS203035) One 1.5 mL vial. 3. Thrombin Solution: (Part No. CS203036) One 0.75 mL vial. 4. ITS Media Supplement, 100X: (Part No. CS203037) One 0.5 mL vial. 5. PMA (Phorbol 12-Myristate 13-Acetate): (Part No. CS203029): One 1 mg vial. 6. Calcein-AM: (Part No. CS202541) One 50 µg vial.
Detection method	Fluorescent

Product Information

Components

1. Millicell® µ-Angiogenesis Slides: (Part No. CS203030) Five tissue culture treated slides. Each slide contains 15 wells.
2. Fibrinogen Solution: (Part No. CS203035) One 1.5 mL vial.
3. Thrombin Solution: (Part No. CS203036) One 0.75 mL vial.
4. ITS Media Supplement, 100X: (Part No. CS203037) One 0.5 mL vial.
5. PMA (Phorbol 12-Myristate 13-Acetate): (Part No. CS203029): One 1 mg vial.
6. Calcein-AM: (Part No. CS202541) One 50 µg vial.

Detection method

Fluorescent

Applications
Application	The Millicell μ-Angiogenesis activation assay kits provide a powerful, quantitative platform for real-time monitoring of changes in tubule formation with unprecedented optical resolution.
Key Applications	Activity Assay Cell Culture

Applications

Application

The Millicell μ-Angiogenesis activation assay kits provide a powerful, quantitative platform for real-time monitoring of changes in tubule formation with unprecedented optical resolution.

Key Applications

Activity Assay
Cell Culture

Biological Information
Species Reactivity	Vertebrates Rat Human

Biological Information

Species Reactivity

Vertebrates
Rat
Human

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	1. Millicell® µ-Angiogenesis Slides (Part No. CS203030) should be stored at room temperature until ready to use. 2. Fibrinogen Solution (Part No. CS203035) should be stored at -20ºC and used within six months from the date of receipt. For short-term storage, store at room temperature for up to 2 weeks. Do not store at 4ºC, as Fibrinogen Solutions tend to form aggregates when stored at 4ºC. For long-term storage, aliquot into sterile tubes in a sterile laminar flow hood, freeze on dry ice, and store at -20ºC. 3. Thrombin Solution (Part No. CS203036) should be stored at -20ºC and used within six months from the date of receipt. Unused Thrombin Solution can be stored at 4ºC for up to 1 month. For longer term storage, Thrombin Solution may be stored in aliquots at -20ºC. 4. ITS Media Supplement, 100X (Part No. CS203037) should be stored at -20ºC. Unused portions may also be stored at 4ºC for up to 1 year. Avoid freeze thaw cycles. 5. PMA (Phorbol-12-Myristate-13-Acetate) (Part No. CS203029) is provided as a 1 mg lyophilized powder. To prepare a 1 mg/mL stock, add 1 mL sterile DMSO (not provided). Vortex vigorously. Centrifuge briefly to collect the solution. Unused PMA Solution may be stored at -20ºC in the dark for at least six months. WARNING: Highly irritating to skin and mucous membranes. TUMOR PROMOTER. Read the Material Safety Data Sheet (MSDS) prior to rehydration and use. Gloves and mask should be worn when using this compound. 6. Calcein AM (Part No. CS202541) is provided as a 50 µg lyophilized powder. To prepare 1 mM Calcein AM Stock Solution, briefly centrifuge before opening the tube and then add 40 µL sterile DMSO (not provided). Vortex vigorously. Centrifuge briefly to collect the solution. Unused Calcein AM Solution may be stored in aliquots at –20ºC for up to 1 month.

Storage and Shipping Information

Storage Conditions

1. Millicell® µ-Angiogenesis Slides (Part No. CS203030) should be stored at room temperature until ready to use.
2. Fibrinogen Solution (Part No. CS203035) should be stored at -20ºC and used within six months from the date of receipt. For short-term storage, store at room temperature for up to 2 weeks. Do not store at 4ºC, as Fibrinogen Solutions tend to form aggregates when stored at 4ºC. For long-term storage, aliquot into sterile tubes in a sterile laminar flow hood, freeze on dry ice, and store at -20ºC.
3. Thrombin Solution (Part No. CS203036) should be stored at -20ºC and used within six months from the date of receipt. Unused Thrombin Solution can be stored at 4ºC for up to 1 month. For longer term storage, Thrombin Solution may be stored in aliquots at -20ºC.
4. ITS Media Supplement, 100X (Part No. CS203037) should be stored at -20ºC. Unused portions may also be stored at 4ºC for up to 1 year. Avoid freeze thaw cycles.
5. PMA (Phorbol-12-Myristate-13-Acetate) (Part No. CS203029) is provided as a 1 mg lyophilized powder. To prepare a 1 mg/mL stock, add 1 mL sterile DMSO (not provided). Vortex vigorously. Centrifuge briefly to collect the solution. Unused PMA Solution may be stored at -20ºC in the dark for at least six months. WARNING: Highly irritating to skin and mucous membranes. TUMOR PROMOTER. Read the Material Safety Data Sheet (MSDS) prior to rehydration and use. Gloves and mask should be worn when using this compound.
6. Calcein AM (Part No. CS202541) is provided as a 50 µg lyophilized powder. To prepare 1 mM Calcein AM Stock Solution, briefly centrifuge before opening the tube and then add 40 µL sterile DMSO (not provided). Vortex vigorously. Centrifuge briefly to collect the solution. Unused Calcein AM Solution may be stored in aliquots at –20ºC for up to 1 month.

Packaging Information
Material Size	1 Kit

Packaging Information

Material Size

1 Kit

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Millicell® µ-Angiogenesis Activation Assay

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