Accessory box for EV262 CellASIC ONIX Microfluidic System

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Ch	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Purified

Polyclonal Antibody

Description
Catalogue Number	ABN22
Description	Anti-UCH-L1 Antibody
Alternate Names	Ubiquitin carboxyl-terminal hydrolase isozyme L1 UCH-L1 Neuron cytoplasmic protein 9.5 PGP 9.5 PGP9.5 Ubiquitin thioesterase L1
Background Information	Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-termial glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and thier tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5.

Description

Catalogue Number

ABN22

Description

Anti-UCH-L1 Antibody

Alternate Names

Ubiquitin carboxyl-terminal hydrolase isozyme L1
UCH-L1
Neuron cytoplasmic protein 9.5
PGP 9.5
PGP9.5
Ubiquitin thioesterase L1

Background Information

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-termial glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and thier tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5.

Product Information
Format	Purified
Control	PC12 cell lysate
Presentation	Purified chicken polyclonal in PBS containing 0.05% sodium azide and 10% glycerol.

Product Information

Format

Purified

Control

PC12 cell lysate

Presentation

Purified chicken polyclonal in PBS containing 0.05% sodium azide and 10% glycerol.

Applications
Application	This Anti-UCH-L1 Antibody is validated for use in WB for the detection of UCH-L1.
Key Applications	Western Blotting

Applications

Application

This Anti-UCH-L1 Antibody is validated for use in WB for the detection of UCH-L1.

Key Applications

Western Blotting

Biological Information
Immunogen	Histidine-tagged recombinant protein corresponding to human UCH-L1.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Chicken
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_004172
Entrez Gene Summary	The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
Gene Symbol	UCHL1
Purification Method	IgY purified
UniProt Number	P09936
UniProt Summary	FUNCTION: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. CATALYTIC ACTIVITY: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). SUBUNIT STRUCTURE: Monomer. Homodimer. Interacts with SNCA By similarity. Interacts with COPS5. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. PTM: O-glycosylated (By similarity). INVOLVEMENT IN DISEASE: Defects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia. MISCELLANEOUS: Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles. In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8. SEQUENCE SIIMILARITIES: Belongs to the peptidase C12 family. Caution Ref.8 reports the association of mutation Ile93Met with Parkinson disease. However, according to Ref.26 this association is uncertain and UCHL1 is not a susceptibility gene for Parkinson disease. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=122 nM for Ub-AMC KM=1.20 µM for ubiquitin ethyl ester Vmax=0.47 µmol/min/mg enzyme toward Ub-AMC Vmax=25 µmol/min/mg enzyme toward ubiquitin ethyl ester SEQUENCE CAUTION: The sequence CAA28443.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Molecular Weight	~26 kDa observed

Biological Information

Immunogen

Histidine-tagged recombinant protein corresponding to human UCH-L1.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Chicken

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_004172

Entrez Gene Summary

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.

Gene Symbol

UCHL1

Purification Method

IgY purified

UniProt Number

P09936

UniProt Summary

FUNCTION: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

CATALYTIC ACTIVITY: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

SUBUNIT STRUCTURE: Monomer. Homodimer. Interacts with SNCA By similarity. Interacts with COPS5.

SUBCELLULAR LOCATION: Cytoplasm.

TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

PTM: O-glycosylated (By similarity).

INVOLVEMENT IN DISEASE: Defects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.

MISCELLANEOUS: Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles. In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.

SEQUENCE SIIMILARITIES: Belongs to the peptidase C12 family.

Caution Ref.8 reports the association of mutation Ile93Met with Parkinson disease. However, according to Ref.26 this association is uncertain and UCHL1 is not a susceptibility gene for Parkinson disease.

BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:

KM=122 nM for Ub-AMC

KM=1.20 µM for ubiquitin ethyl ester

Vmax=0.47 µmol/min/mg enzyme toward Ub-AMC

Vmax=25 µmol/min/mg enzyme toward ubiquitin ethyl ester

SEQUENCE CAUTION: The sequence CAA28443.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Molecular Weight

~26 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in PC12 cell lysate. Western Blot Analysis: 0.2 µg/mL of this antibody detected UCH-L1 on 10 µg of PC12 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected UCH-L1 on 10 µg of PC12 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Accessory box for EV262 CellASIC ONIX Microfluidic System

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