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PDGF-BB, Human recombinant

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货号：GF149

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Source Purity
CULT Human E. coli Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of PDGF-BB.
Description
Catalogue Number GF149
Replaces GF018
Brand Family Chemicon®
Trade Name
  • Chemicon
Description PDGF-BB, recombinant human
Alternate Names
  • Platelet Derived Growth Factor-BB
Background Information Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
References
Product Information
Formulation
Presentation Sterile filtered solution lyophilized from 10 mM HOAc.
Applications
Application The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.
Key Applications
  • Cell Culture
Application Notes For most in vitro applications, PDGF-BB exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): fibroblasts, connective tissue.
Biological Information
Purity Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of PDGF-BB.
Source E. coli
Specific Activity Human PDGF-BB is fully biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells is between 6-12 ng/mL.
Gene Symbol
  • PDGFB
  • PDGF2
  • SIS
UniProt Number
Stem Cell Type
  • Neural Stem Cells
  • Mesenchymal Stem Cells
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-BB should be stored in undiluted aliquots at -20°C for up to six months.
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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