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Recombinant Human Neurotrophin-3

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货号：GF031

品牌：Millipore 密理博

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Replacement Information

重要规格表

Source Purity
E.coli Greater than 98% by SDS-PAGE analysis
Description
Catalogue Number GF031
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Neurotrophin 3
Overview NT-3 is a potent neurotrophic factor which supports the growth and survivability of nerve and/or glial cells. The active form of human NT-3 is a dimer, formed by two 13.6 kDa polpeptide monomers (240 total amino acid residues), which is held together by strong hydrophobic interactions.
Alternate Names
  • NT3
References
Product Information
Presentation Lyophilized protein with no additives.
Applications
Application The Neurotrophin-3 protein (or NT-3 protein) is a potent neurotrophic factor which supports the growth & survivability of nerve and/or glial cells.
Application Notes Concentration range for most in vitro applications is 20-50 ng/mL. Responding cells are (partial list): Nerve, glial cells.
Biological Information
Purity Greater than 98% by SDS-PAGE analysis
Source E.coli
Specific Activity Milliprore's human NT-3 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cultures was found to be <= 50 ng/mL.
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
Gene Symbol
  • NTF3
  • NT3
  • NGF2
  • HDNF
  • MGC129711
  • NGF-2
  • NT-3
UniProt Number
UniProt Summary FUNCTION: SwissProt: P20783 # Seems to promotes the survival of visceral and proprioceptive sensory neurons.
SIZE: 257 amino acids; 29355 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Brain and peripheral tissues.
SIMILARITY: SwissProt: P20783 ## Belongs to the NGF-beta family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute with sterile water to a concentration of 100 ng/uL. This solution can be diluted into water or other buffered solutions. Reconstitute NT-3 should be stored in undiluted aliquots at -20°C for up to 6 months.
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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