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Recombinant Human GM-CSF

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货号：GF004

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Source Purity
CULT Human E.coli Greater than 98% by SDS-PAGE analysis.
Description
Catalogue Number GF004
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Granulocyte-Macrophage Colony-Stimulating Factor Protein, Recombinant human
Overview eGranulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. Human GM-CSF is a 14 kDa protein containing 128 amino acids residues.
Alternate Names
  • GM-CSF
Background Information GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
References
Product Information
Presentation The sterile filtered solution was lyophilized from 10mM Sodium Citrate, pH 3.5.
Applications
Application The Granulocyte Macrophage Colony Stimulating Factor protein (GM-CSF protein) is a potent species-specific stimulator of bone marrow cells.
Key Applications
  • Cell Culture
Application Notes For most in vitro applications, human GM-CSF exerts its biological activity in the concentration range of 0.05 to 0.5 ng/mL. Responding cells are (partial list): Certain BM precursor cells.

Optimal working dilutions must be determined by end user.
Biological Information
Purity Greater than 98% by SDS-PAGE analysis.
Source E.coli
Specific Activity Human GM-CSF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <= 0.1 ng/mL, corresponding to a specific activity of >=1 x 10<sup>7</sup> units/mg.
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Gene Symbol
  • IL3
  • MGC79399
  • MCGF
  • MULTI-CSF
  • MGC79398
  • IL-3
UniProt Number
UniProt Summary FUNCTION: SwissProt: P08700 # This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
SIZE: 152 amino acids; 17233 Da
SUBUNIT: Monomer.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Activated T-cells, mast cells, natural killer cells.
SIMILARITY: SwissProt: P08700 ## Belongs to the IL-3 family.
Stem Cell Type
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized powder, though stable at room temperature, is best stored at -20ºC. After a quick spin, reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions and stored at 2-8ºC for 1 week or -20ºC for future use. After reconstitution maintain at -20ºC in undiluted aliquots for up to six months.
Packaging Information
Material Size 20 µg
Transport Information
Supplemental Information
Specifications

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