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Human recombinant Histone H2AZ-H2B dimers

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货号：14-1053

品牌：Millipore 密理博

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重要规格表

Key Applications
ACT
Description
Catalogue Number 14-1053
Brand Family Upstate
Trade Name
  • Upstate
Description Human recombinant histone H2AZ-H2B dimers
Overview Human recombinant histone H2AZ-H2B dimers expressed in E Coli. as fusion proteins with either an N-terminal FLAG® or 6xHis epitopes, and purified by Fast Protein Liquid Chromatography (FPLC).
Background Information The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The H2AZ histone variant replaces conventional H2A in a subset of nucleosomes and plays an essential role in transcriptional regulation, autosilencing, gene silencing, and genome stability. The effects of histone variants and histone modifications on chromatin structure and gene expression can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin and essential tools, such as histone proteins and appropriate enzymes to assemble chromatin in vitro.
References
Product Information
Presentation In buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 1 M NaCl, 0.5 mM PMSF and 1 mM DTT
Applications
Application Human recombinant histone H2AZ-H2B dimers expressed in E Coli as fusion proteins with either an N-terminal FLAG® or 6xHis epitopes, & purified by Fast Protein Liquid Chromatography (FPLC)& verified by mass spectrometry.
Key Applications
  • Activity Assay
Application Notes Suitable for in vitro chromatin assembly and enzyme activity assays
Biological Information
Concentration 1 µg/µL
Host E. coli
Purity >98% purity by SDS PAGE
Source Human
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable at -80ºC for 1 year from date of receipt.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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