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Human recombinant Histone H2A-H2B dimers

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货号：14-1052

品牌：Millipore 密理博

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重要规格表

Key Applications
ACT
Description
Catalogue Number 14-1052
Brand Family Upstate
Trade Name
  • Upstate
Description Human recombinant histone H2A-H2B dimers
Overview Human recombinant histone H2A-H2B dimers expressed in E Coli. as fusion proteins with either an N-terminal FLAG® or 6xHis epitopes, and purified by Fast Protein Liquid Chromatography (FPLC).
Background Information The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The nucleosome is the first order of DNA compaction in the nucleus, and is the basic unit of chromatin. The structure of chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of H2A, H2B, H3, and H4. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences, and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin, and essential tools such as histone proteins and appropriate enzymes, to assemble chromatin in vitro.
References
Product Information
Presentation In buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 1 M NaCl, 0.5 mM PMSF and 1 mM DTT
Applications
Application Human recombinant histone H2A-H2B dimers expressed in E Coli as fusion proteins with either an N-terminal FLAG or 6xHis epitopes & purified by Fast Protein Liquid Chromatography (FPLC).
Key Applications
  • Activity Assay
Application Notes Suitable for in vitro chromatin assembly and enzyme activity assays
Biological Information
Concentration 1 µg/µL
Host E. coli
Purity >98% purity by SDS PAGE
Source Human
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable at -80ºC for 1 year from date of receipt.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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