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Hu Ghrelin (Tot) ELISA (BULKPKG) C-Packs

市场价：
48.00
促销价：
¥0.00

货号：EZGRT-89BK

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
Ghrelin (Total) H ELISA Fluorescent, Colorimetric
Description
Catalogue Number EZGRT-89BK
Description Human Ghrelin (total) ELISA- Bulk
Background Information Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach.
References
Product Information
Detection method Fluorescent Colorimetric
Precision, %
  • Inter-assay: Serum: 6.2–7.8% Plasma: 5.2–7.7%
  • Intra-assay: Serum: 0.9–1.3% Plasma: 1.1–1.9%
Applications
Application This Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.
Key Applications
  • ELISA
Application Notes Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Biological Information
Specificity Human Ghrelin (active) 80% Des-Octanoyl Human Ghrelin 100% Canine Ghrelin (active) 70% Porcine Ghrelin (active) not detectable Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
Species Reactivity
  • Human
Analytes Available
  • Ghrelin (Total)
Entrez Gene Number
Entrez Gene Summary Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
Gene Symbol
  • GHRL
  • ghrelin
  • obestatin
  • MTLRP
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
SIZE: 117 amino acids; 12911 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
Accuracy
  • Serum: (Active Ghrelin) 88–91%
  • (Des-octanoyl Ghrelin) 101–107%
  • Plasma: (Active Ghrelin) 84–87%
  • (Des-octanoyl Ghrelin) 112–119%
Physicochemical Information
Sensitivity
  • 30 pg/mL (20 µL sample size)
Linearity of Dilution Serum: 95.7–104.7% Plasma: 95–117%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Availability by Geography
  • This product is not available for sale in Japan.
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size Ten 96-well strip plates
Material Package This product consists of ten Human Ghrelin (total) ELISA kits.
Transport Information
Supplemental Information
Specifications

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